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IL-10 ELISA Kit

IL10 Reaktivität: Maus Colorimetric Sandwich ELISA 3.12-200 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN365237
  • Target Alle IL-10 (IL10) ELISA Kits anzeigen
    IL-10 (IL10) (Interleukin 10 (IL10))
    Reaktivität
    • 20
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    3.12-200 pg/mL
    Untere Nachweisgrenze
    3.12 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    For the quantitative determination of mouse interleukin 10 (IL-10) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
    Proben
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of mouse IL-10.
    Kreuzreaktivität (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivität
    0.78 pg/mL
    Bestandteile
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Applikationshinweise
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Kommentare

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Aufbereitung der Reagenzien
    • Biotin-antibody (1×) - Centrifuge the vial before opening.
      Biotin-antibody requires a 100-fold dilution. The suggested dilution is 10µL of Biotin-antibody + 990µL of Biotin-antibody Diluent.
    • HRP-avidin (1×) - Centrifuge the vial before opening.
      HRP-avidin requires a 100-fold dilution. The suggested dilution is 10µL of HRP-avidin + 990µL of HRP-avidin Diluent.
    • Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20mL of Wash Buffer Concentrate (25×) into deionized or distilled water to prepare 500mL of Wash Buffer (1×).
    • Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
      Reconstitute the Standard with 1ml of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
      Pipette 250µL of Sample Diluent into each tube. Use the stock solution to produce a 2-fold dilution series. Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard (0ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25°C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction. Avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL when pipetting.
    • It is recommended to use distilled water to prepare reagents and samples. Using contaminated water or container for reagent preparation will influence detection result.
    Testpräzision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handhabung
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Haltbarkeit
    6 months
  • Liu, You, Zhang, Ma, Dong, Chen, Jiang, Yun: "The Hsp90 inhibitor 17-DMAG decreases infection of porcine circovirus type 2 in mice." in: Microbial pathogenesis, Vol. 109, pp. 248-252, (2018) (PubMed).

    Xu, Guo, Zhao, He, Wang, Lin, Wang, Liu: "Punicalagin, a PTP1B inhibitor, induces M2c phenotype polarization via up-regulation of HO-1 in murine macrophages." in: Free radical biology & medicine, Vol. 110, pp. 408-420, (2018) (PubMed).

    Wang, Tan, Jin, Sun, Chen, Tan, Su, Shi: "Non-cultured dermal-derived mesenchymal cells attenuate sepsis induced by cecal ligation and puncture in mice." in: Scientific reports, Vol. 5, pp. 16973, (2016) (PubMed).

    Wu, Fang, Shen, Liu: "SP600125 promotes resolution of allergic airway inflammation via TLR9 in an OVA-induced murine acute asthma model." in: Molecular immunology, (2015) (PubMed).

    Zhang, Peng, Cheng, Shi, Zhang, Xu: "Paeoniflorin Atttenuates Amyloidogenesis and the Inflammatory Responses in a Transgenic Mouse Model of Alzheimer's Disease." in: Neurochemical research, Vol. 40, Issue 8, pp. 1583-92, (2015) (PubMed).

    Li, Liu, Peng, Liu, Zhao, Feng, Han, Huang, Luo, Li, Yue, Cheng, Huang, Luo: "NMDA Receptor Antagonist Attenuates Bleomycin-Induced Acute Lung Injury." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125873, (2015) (PubMed).

    Wang, Zhang, Ma, He, Zhe, Zhou: "Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice." in: Drug design, development and therapy, Vol. 9, pp. 3163-78, (2015) (PubMed).

    Guo, Wang, Zhou, Wu, Ma, Liu, Huang, Qin: "Lentiviral Vector-Mediated FoxO1 Overexpression Inhibits Extracellular Matrix Protein Secretion under High Glucose Conditions in Mesangial Cells." in: Journal of cellular biochemistry, (2015) (PubMed).

    Zong, Zeng, Fang, Peng, Zou, Gao, Zhao: "The effects of ?-zearalanol on the proliferation of bone-marrow-derived mesenchymal stem cells and their differentiation into osteoblasts." in: Journal of bone and mineral metabolism, (2015) (PubMed).

    Sakumoto, Hayashi, Saito, Kanahara, Kizaki, Iga: "Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn." in: The Journal of reproduction and development, (2015) (PubMed).

    Zhao, Zhong, Qu, Xie, Cao, Li, Yang, Varghese, Moorhead, Chen, Ruan: "Chronic inflammation aggravates metabolic disorders of hepatic fatty acids in high-fat diet-induced obese mice." in: Scientific reports, Vol. 5, pp. 10222, (2015) (PubMed).

    Wang, Zhong: "Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice." in: International journal of clinical and experimental medicine, Vol. 8, Issue 9, pp. 16536-42, (2015) (PubMed).

    Huang, Zhao, Ran, Wang: "Increased expression of herpesvirus entry mediator in 1,25-dihydroxyvitamin D3-treated mouse bone marrow-derived dendritic cells promotes the generation of CD4⁺CD25⁺Foxp3⁺ regulatory T cells." in: Molecular medicine reports, Vol. 9, Issue 3, pp. 813-8, (2014) (PubMed).

    Shen, Fang, Wu, He, Ding, Liu: "Repeated inhalation of sevoflurane inhibits airway inflammation in an OVA-induced mouse model of allergic airway inflammation." in: Respirology (Carlton, Vic.), (2014) (PubMed).

  • Target Alle IL-10 (IL10) ELISA Kits anzeigen
    IL-10 (IL10) (Interleukin 10 (IL10))
    Andere Bezeichnung
    Interleukin 10 (IL-10) (IL10 Produkte)
    Synonyme
    CSIF ELISA Kit, GVHDS ELISA Kit, IL-10 ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Il-10 ELISA Kit, IL10X ELISA Kit, interleukin 10 ELISA Kit, IL10 ELISA Kit, Il10 ELISA Kit
    Hintergrund
    Synonyms: CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
    UniProt
    P18893
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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