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Vitamin D-Binding Protein CLIA Kit

GC Reaktivität: Human Chemiluminescent Sandwich ELISA 1.37 ng/mL - 1000 ng/mL Plasma, Serum
Produktnummer ABIN3160692
  • Target Alle Vitamin D-Binding Protein (GC) CLIA Kits anzeigen
    Vitamin D-Binding Protein (GC)
    Reaktivität
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.37 ng/mL - 1000 ng/mL
    Untere Nachweisgrenze
    1.37 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of DBP in human serum, plasma.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität

    This assay has high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP).
    No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.

    Kreuzreaktivität (Details)
    No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.
    Sensitivität
    0.59 ng/mL
    Bestandteile
    • Pre-coated, ready to use black 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • Substrate A
    • Substrate B
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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  • Applikationshinweise
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Kommentare

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Probenmenge
    100 μL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vitamin D Binding Protein (DBP) level in the sample or standard.,
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 8,000 ng/mL. Firstly dilute the stock solution to 1,000 ng/mL and the diluted standard serves as the highest standard (1,000 ng/mL). Then prepare 7 tubes containing 0.6 mL Standard Diluent and use the diluted standard to produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000 ng/mL, 333.33 ng/mL, 111.11 ng/mL, 37.04 ng/mL, 12.35 ng/mL, 4.12 ng/mL, 1.37 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with Assay Diluent A and B, respectively (1:100).
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. Substrate working Solution - Mix the substrate A and B by the ratio of 99:1 to make the substrate working solution. Mix thoroughly. For example, prepare 1,000μL Substrate working Solution with 990μL Substrate A + 10μL Substrate B.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. Prepare Substrate working Solution within 15 minutes before assay.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Lagerung
    4 °C
    Informationen zur Lagerung
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Haltbarkeit
    6 months
  • Tannetta, Redman, Sargent: "Investigation of the actin scavenging system in pre-eclampsia." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 172, pp. 32-5, (2013) (PubMed).

  • Target Alle Vitamin D-Binding Protein (GC) CLIA Kits anzeigen
    Vitamin D-Binding Protein (GC)
    Andere Bezeichnung
    Vitamin D Binding Protein (DBP) (GC Produkte)
    Synonyme
    DBP CLIA Kit, DBP/GC CLIA Kit, GRD3 CLIA Kit, VDBG CLIA Kit, VDBP CLIA Kit, zgc:110389 CLIA Kit, zgc:92753 CLIA Kit, GC CLIA Kit, Gc CLIA Kit, DBP02 CLIA Kit, VDB CLIA Kit, Vdbp CLIA Kit, VTDB CLIA Kit, gc CLIA Kit, GC, vitamin D binding protein CLIA Kit, D-box binding PAR bZIP transcription factor CLIA Kit, D site albumin promoter binding protein CLIA Kit, group-specific component (vitamin D binding protein) CLIA Kit, group specific component CLIA Kit, group-specific component (vitamin D binding protein) S homeolog CLIA Kit, GC CLIA Kit, Dbp CLIA Kit, gc CLIA Kit, Gc CLIA Kit, gc.S CLIA Kit
    UniProt
    P02774
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
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