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These extracts are diluted down to 0.625 μg/well and assayed using the TransAM AP-1 c-Jun Kit. The ratio of the signals from the stimulated over the unstimulated cells must be above 2. Lot No.18715036 was tested using K-562 cells. It was developed for 4 minutes and gave a ratio of 6.4 (Figure 1). The basal level of c-Jun expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AP-1 c-Jun Kits are also tested for specificity in detecting c-Jun activation. TransAM AP-1 c-Jun assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AP-1 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AP-1 binding.