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Rapid Ratte Monoclonal Antibody Isotyping Kit

IsoT Reaktivität: Ratte Cell Culture Supernatant, Purified Protein
Produktnummer ABIN2860629
  • Reaktivität
    Ratte
    Untere Nachweisgrenze
    100 ng/mL
    Applikation
    Isotyping (IsoT)
    Verwendungszweck
    5 minute determination of 6 Rat Ig heavy chain isotypes
    Proben
    Cell Culture Supernatant, Purified Protein
    Kreuzreaktivität (Details)
    There is no cross-reactivity among each isotype.
    Produktmerkmale
    The Rapid Rat Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination.
    Acceptable sample types include hybridoma culture supernatant and purified monoclonal antibody.
    Ascites fluid is not recommended since it usually contains multiple immunoglobulins.
    However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid.
    Bestandteile
    The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2a, IgG2b, IgG2c and the other cassette is for determining Kappa, Lambda, IgA and IgM.
    Both strips contain a control line.
  • Kommentare

    1. Invalid result: no red line at ""C"".
    2. Negative result: only one red line at ""C"".
    3. Positive results:
    a). Single monoclonal antibody: In addition to the positive control ""C"" line, only one heavy chain and one light chain will appear.
    b). Multiple monoclonal antibodies: In addition to the positive control line, more than one heavy chain and/or one light chain will appear.
    The line intensity can be used to judge the dominant Isotypes.

    Probenmenge
    100 µL
    Testdauer
    5 min
    Protokoll
    1) Place the cassettes in horizontal plane.
    2) Add 80-100 μL antibody solution directly to the Sample Well. Wait for 5 minutes.
    3) A red test line at the location corresponding to antibody-specific isotype and a red control line at ""C' position will appear within 1-5 minutes.
    NOTE: Do not read after 20 minutes.
    NOTE: If the color of tissue culture medium causes high background, 40-60 μL PBS can be chased to clear the background.
    Aufbereitung der Proben

    Cell Culture Supernatant: Recommended dilution is 1:10-1:100 in PBS.
    Purified Antibody: Dilute samples to 0.5 - 2 μg/mL in PBS
    Ascites: Ascites fluid is not recommended since it usually contains multiple Igs. However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid. The recommended dilution is 1:10,000 in PBS.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Promptly return unused strips in sealed bag and keep dry.
    Lagerung
    RT
    Informationen zur Lagerung
    Store the strips in their original package at room temperature.
    This product is stable for up to 18 months from the certified date. Freezing the strips is not recommended.
    Haltbarkeit
    18 months
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