Apoptosis Detection Kit (Annexin-V-FITC / PI)

Details zu Produkt Nr. ABIN2746064, Anbieter: Anmelden zum Anzeigen
Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin A5 L homeolog
  • ANXA5
  • anxa5b
  • anxa5
  • Anxa5
  • anxa5.L
Alternativen
Human Annexin V ELISA Kit
Reaktivität
Human
17
16
15
14
13
8
7
7
4
2
2
2
2
1
1
1
1
Konjugat
FITC
Applikation
Flow Cytometry (FACS)
Optionen
Hersteller
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Verwendungszweck This Kit is designed to rapidly analyse different stages of apoptosis and cell death in single cell suspensions.
Produktmerkmale Annexin V binds specifically to phosphatidylserine (PS) which is located to the inner side of the cytoplasmic membrane in intact cells. But after initiation of apoptosis PS becomes rapidly translocated to the outer side of the cytoplasmic membrane where it is thought to play an important role in macrophage recognition, thus allowing the apoptotic cells to become rapidly phagocytosed. The binding of annexin V to PS is Ca2+ -dependent and therefore, a specific Ca2+ -containing reaction buffer is needed during the binding process. At low PS concentrations a binding ratio of eight annexin V molecules to one PS has been reported making annexin V- conjugates ideal for identifying membrane changes associated with early apoptotic events. In late apoptosis/necrosis cells loose their membrane integrity which allows further annexin V binding to PS located to the inner cell membrane. Therefore, Annexin V Apoptosis Detection Kit contains a viability dye such as propidium iodide (PI). This DNAintercalating dye can only pass membranes which lost their integrity and therefore, allow the discrimination of early apoptotic cells (Annexin V+ /PI-) from late apoptotic/necrotic cells (Annexin V+ /PI+).
Bestandteile 500 μl Annexin V FITC conjugate, (with 1% BSA / 0.1% NaN3 as preservative)
500 μl propidium iodide [20 μg/ml], (w/o preservative)
10 ml Annexin V binding buffer (10fold concentrate), (w/o preservative)
Benötigtes Material
  • Flow cytometer or fluorescence microscope
  • Microcentrifuge
  • Microcentrifuge tubes
  • Adjustable pipettors
  • Pipette tips
  • Gloves
  • Deionized or destilled water
  • PBS and/or culture medium
Andere Bezeichnung Annexin-V (ANXA5 ELISA Kit Abstract)
Testdurchführung
  1. Dilute an appropriate amount of 10fold Annexin V binding buffer with deionized or
    distilled water in 1:10 ratio (mix 1 part 10x buffer with 9 parts water)
    2. Wash cells with culture medium or PBS
    3. Resuspend 104 - 106 cells in 90 μL of diluted (1 fold) Annexin V binding buffer.
    4. Add 5 μL of Annexin V conjugate and 5 μL of propidium iodide solution.
    5. Incubate for 20 minutes in the dark.
    6. Add 400 μL of Annexin V binding buffer (1fold).
    7. Centrifuge at 400 x g for 5 minutes.
    8. Resuspend cells in 200 μL Annexin V binding buffer (1fold) and analyze sample by flow cytometry or fluorescence microscopy.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Upon arrival store all reagents in the dark at 2 - 8 °C. If stored properly all reagents will be stable for 12 months
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for Apoptosis Detection Kit (Annexin-V-FITC / PI) (ABIN2746064) 1. viable cells (double-negative)
2. early apoptotic cells (annexin V-positive...
Produkt verwendet in: Lim, Maubach, Sokolova, Feige, Diezko, Buchbinder, Backert, Schlüter, Lavrik, Naumann: "Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-κB and caspase-8-dependent apoptotic cell death." in: Cell death and differentiation, Vol. 24, Issue 9, pp. 1621-1631, 2017 (PubMed).

Honisch, Yu, Liu, Alesutan, Towhid, Tsapara, Schleicher, Handgretinger, Stournaras, Lang: "Chorein addiction in VPS13A overexpressing rhabdomyosarcoma cells." in: Oncotarget, Vol. 6, Issue 12, pp. 10309-19, 2015 (PubMed).