VWF ELISA Kit

Produktnummer ABIN2703573

Produktdetails VWF ELISA Kit

Target: VWF (Von Willebrand Factor (VWF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.13-120 ng/mL

Untere Nachweisgrenze: 0.13 ng/mL

Applikation: ELISA

Verwendungszweck: Human VWF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human vWF. Other species not determined.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für VWF ELISA Kit (ABIN2703573)

Małkiewicz, Słomiński, Skrzypkowska, Siebert, Gutknecht, Myśliwska: "The GA genotype of the -1154 G/A (rs1570360) vascular endothelial growth factor (VEGF) is protective against hypertension-related chronic kidney disease incidence." in: Molecular and cellular biochemistry, Vol. 418, Issue 1-2, pp. 159-65, (2016) (PubMed).

Małkiewicz, Myśliwska, Skrzypkowska, Słomiński, Siebert, Gutknecht: "Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease." in: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 38, Issue 5, pp. 469-75, (2016) (PubMed).

Małkiewicz, Skrzypkowska, Słomiński, Siebert, Gutknecht, Myśliwska: "The GG genotype of the -152 G/A vascular endothelial growth factor (VEGF) polymorphism predisposes to hypertension-related chronic kidney disease." in: Molecular biology reports, Vol. 43, Issue 9, pp. 967-75, (2016) (PubMed).

Ma, Su, Zhang, Ling, Yin, Bai, Ruan: "The co-influence of VWD type 2B/2M mutations in the A1 domain and platelet GPIbα on the rate of cleavage to VWF by ADAMTS13." in: Thrombosis research, Vol. 136, Issue 5, pp. 987-95, (2015) (PubMed).

Zhai, Chen, Gao, Ma, Chen, Yu, Li, Liu, Xiang, Xie, Yang: "Inhibition of the NF-κB pathway by R65 ribozyme gene via adeno-associated virus serotype 9 ameliorated oxidized LDL induced human umbilical vein endothelial cell injury." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9912-21, (2015) (PubMed).

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: vWF (VWF Produkte)

Hintergrund: Gene Names: VWF F8VWF
Protein names: von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]

Gen-ID: 7450

UniProt: P04275