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PD-1 ELISA Kit

PDCD1 Reaktivität: Human Colorimetric Sandwich ELISA 20-6.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703394
  • Target Alle PD-1 (PDCD1) ELISA Kits anzeigen
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Reaktivität
    • 4
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    20-6.000 pg/mL
    Untere Nachweisgrenze
    20 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human PD-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects human PD-1. Other species not determined yet.
    Sensitivität
    20 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Yanaba, Hayashi, Yoshihara, Nakagawa: "Serum levels of soluble programmed death-1 and programmed death ligand-1 in systemic sclerosis: Association with extent of skin sclerosis." in: The Journal of dermatology, Vol. 43, Issue 8, pp. 954-7, (2016) (PubMed).

  • Target Alle PD-1 (PDCD1) ELISA Kits anzeigen
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Andere Bezeichnung
    PD-1 (PDCD1 Produkte)
    Synonyme
    CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit
    Hintergrund
    Gene Names: PDCD1 PD1
    Protein names: Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279)
    Gen-ID
    5133
    UniProt
    Q15116
    Pathways
    Cancer Immune Checkpoints
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