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TNF alpha ELISA Kit

TNF alpha Reaktivität: Ratte Colorimetric Cell Culture Cells, Plasma, Serum
Produktnummer ABIN2648838
  • Target Alle TNF alpha ELISA Kits anzeigen
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Reaktivität
    • 14
    • 11
    • 10
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Applikation
    ELISA
    Verwendungszweck
    Rat TNF Alpha (TNF-α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of rat γ-interferon (TNF-α ) concentrations in cell culture supernates, serum, and plasma.
    Proben
    Serum, Plasma, Cell Culture Cells
    Analytische Methode
    Quantitative
    Sensitivität
    15 pg/ml
    Produktmerkmale
    Rat TNF Alpha (TNF-α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Testdauer
    3.5 h
    Plattentyp
    Pre-coated
    Protokoll
    Rat TNF Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF Alpha (TNF-α) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF Alpha (TNF-α) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF Alpha (TNF-α) is added to the wells and binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF Alpha (TNF-α) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF Alpha (TNF-α) standard dilutions and TNF Alpha (TNF-α) sample concentration determined.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle TNF alpha ELISA Kits anzeigen
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Andere Bezeichnung
    TNF-alpha (TNF alpha Produkte)
    Synonyme
    DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, RATTNF ELISA Kit, Tnfa ELISA Kit, tnf ELISA Kit, TNF-a ELISA Kit, TNFalpha ELISA Kit, Tnfsf1a ELISA Kit, TNFa ELISA Kit, cTNF ELISA Kit, Tnf-alpha ELISA Kit, tnfa-like ELISA Kit, TNF-ALPHA ELISA Kit, dif ELISA Kit, tnfa ELISA Kit, xtnf ELISA Kit, tnfsf2 ELISA Kit, tnf-alpha ELISA Kit, Cachectin ELISA Kit, tumor necrosis factor ELISA Kit, tumor necrosis factor b (TNF superfamily, member 2) ELISA Kit, tumor necrosis factor alpha ELISA Kit, tumor necrosis factor a (TNF superfamily, member 2) ELISA Kit, TNF ELISA Kit, Tnf ELISA Kit, tnf ELISA Kit, tnfb ELISA Kit, tnf-alpha ELISA Kit, LOC103694380 ELISA Kit, tnfa ELISA Kit
    Pathways
    NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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