Musculin (MSC) ELISA Kit

Details zu Produkt Nr. ABIN2114604, Anbieter: Anmelden zum Anzeigen
Antigen
  • Msc
  • MSC
  • abf1
  • myor
  • abf-1
  • ABF-1
  • ABF1
  • MYOR
  • bHLHa22
  • MyoR
  • musculin
  • putative musculin
  • msc
  • musculin
  • Smp_022610
  • MSC
  • Msc
Reaktivität
Human, Maus
Alternativen
Kits mit alternativen Reaktivitäten:
2
2
Methodentyp
Cell ELISA
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Proben Cell Culture Cells
Analytische Methode Quantitative
Nachweismethode Colorimetric
Produktmerkmale Assay Type: Cell-Based
Andere Bezeichnung Musculin (MSC ELISA Kit Abstract)
Hintergrund Synonyms: Musculin, Activated B-cell factor 1, ABF-1, MSC, ABF1
Gene Symbol: MSC
Gen-ID 9242
UniProt O60682
Plattentyp Uncoated
Testdurchführung
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles
Lagerung 4 °C
Informationen zur Lagerung Store at 4 °C for frequent use, at -20°C for infrequent use.
Haltbarkeit 6 months
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