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LUM ELISA Kit

LUM Reaktivität: Maus AA 19-338 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Produktnummer ABIN1889412
  • Target Alle LUM ELISA Kits anzeigen
    LUM (Lumican (LUM))
    Bindungsspezifität
    AA 19-338
    Reaktivität
    • 5
    • 2
    • 2
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10.000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Lumican
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: Q19-N338
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q19-N338
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

    Tissue Specificity: Cornea and other tissues.

    Plattentyp
    Pre-coated
    Protokoll
    mouse Lumican ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Lumican has been precoated onto 96-well plates. Standards(NSO, Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Lumican amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Lumican standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Lumican standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 1336, Standard deviation: 65.5, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 3856, Standard deviation: 196.7, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 6635, Standard deviation: 411.4, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 1567, Standard deviation: 91, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 4126, Standard deviation: 264.1, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 7024, Standard deviation: 491.7, CV(%): 7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle LUM ELISA Kits anzeigen
    LUM (Lumican (LUM))
    Andere Bezeichnung
    LUM (LUM Produkte)
    Synonyme
    MGC64527 ELISA Kit, zgc:86661 ELISA Kit, ldc ELISA Kit, slrr2d ELISA Kit, DKFZp468L1116 ELISA Kit, LDC ELISA Kit, SLRR2D ELISA Kit, Ldc ELISA Kit, lumican ELISA Kit, lumican L homeolog ELISA Kit, LUM ELISA Kit, lum.L ELISA Kit, lum ELISA Kit, CpipJ_CPIJ010209 ELISA Kit, Lum ELISA Kit
    Hintergrund

    Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

    Synonyms: Lumican,Keratan sulfate proteoglycan lumican,KSPG lumican,Lum,Lcn, Ldc,

    Full Gene Name: Lumican

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    Gen-ID
    17022
    UniProt
    P51885
    Pathways
    Glycosaminoglycan Metabolic Process
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