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Renin ELISA Kit

REN Reaktivität: Human AA 67-406 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Produktnummer ABIN1889402
  • Target Alle Renin (REN) ELISA Kits anzeigen
    Renin (REN)
    Bindungsspezifität
    AA 67-406
    Reaktivität
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Renin
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: L67-R406
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L67-R406
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the peptidase A1 family.

    Plattentyp
    Pre-coated
    Protokoll
    human Renin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Renin has been precoated onto 96-well plates. Standards(NSO, L67-R406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Renin amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Renin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Renin standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 353, Standard deviation: 19.8, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 643, Standard deviation: 22.51, CV(%): 3.5
    • Sample 3: n=16, Mean(pg/ml): 1204, Standard deviation: 78.26, CV(%): 6.5,
    • Sample 1: n=24, Mean(pg/ml): 287, Standard deviation: 18.1, CV(%): 6.3
    • Sample 2: n=24, Mean(pg/ml): 632, Standard deviation: 32.9, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 1148, Standard deviation: 81.6, CV(%): 7.1
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Wang, Ren: "Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein." in: Hybridoma (2005), Vol. 29, Issue 6, pp. 511-6, (2010) (PubMed).

  • Target Alle Renin (REN) ELISA Kits anzeigen
    Renin (REN)
    Andere Bezeichnung
    REN (REN Produkte)
    Synonyme
    HNFJ2 ELISA Kit, RATRENAA ELISA Kit, RENAA ELISA Kit, Ren1 ELISA Kit, D19352 ELISA Kit, Ren ELISA Kit, Ren-1 ELISA Kit, Ren-A ELISA Kit, Ren1c ELISA Kit, Ren1d ELISA Kit, Rn-1 ELISA Kit, Rnr ELISA Kit, renin ELISA Kit, renin 1 structural ELISA Kit, REN ELISA Kit, Ren ELISA Kit, Ren1 ELISA Kit, ren ELISA Kit
    Hintergrund

    Protein Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

    Background: Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system(RAS). This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.

    Synonyms: Renin,3.4.23.15,Angiotensinogenase,REN,

    Full Gene Name: Renin

    Cellular Localisation: Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    Gen-ID
    5972
    UniProt
    P00797
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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