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SensoLyte® 520 Cathepsin G Assay Kit

FRET Fluorometric
Produktnummer ABIN1882442
  • Target Alle Cathepsin G (CTSG) Kits anzeigen
    Cathepsin G (CTSG)
    Nachweismethode
    Fluorometric
    Applikation
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marke
    SensoLyte®
    Produktmerkmale
    The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte Fluor™ 488/QXL™ 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte Fluor™ 488/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations.
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  • Kommentare

    FRET-based Assay Kit

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Protect Components A and B from light and moisture.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • Wågsäter, Johansson, Fontaine, Vorkapic, Bäcklund, Razuvaev, Mäyränpää, Hjerpe, Caidahl, Hamsten, Franco-Cereceda, Wilbertz, Swedenborg, Zhou, Eriksson: "Serine protease inhibitor A3 in atherosclerosis and aneurysm disease." in: International journal of molecular medicine, Vol. 30, Issue 2, pp. 288-94, (2012) (PubMed).

  • Target
    Cathepsin G (CTSG)
    Andere Bezeichnung
    Cathepsin G (CTSG Produkte)
    Synonyme
    CATG Kit, CG Kit, CTSG Kit, Ctsg Kit, cathepsin G Kit, CTSG Kit, Ctsg Kit, LOC505658 Kit, LOC509956 Kit, ctsg Kit, LOC100053921 Kit, LOC102174648 Kit, LOC100733619 Kit, LOC100154047 Kit
    Hintergrund
    Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.
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