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SensoLyte® Homogeneous Rh110 Caspase-3/7 Assay Kit

FRET Fluorometric
Produktnummer ABIN1882433
  • Target Alle Caspase 3 (CASP3) Kits anzeigen
    Caspase 3 (CASP3)
    Nachweismethode
    Fluorometric
    Applikation
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marke
    SensoLyte®
    Produktmerkmale
    The SensoLyte® Homogeneous Rh110 Capase-3/7 Assay Kit uses (Ac-DEVD)2-Rh110 as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, (Ac-DEVD)2-Rh110 generates the Rh110 fluorophore which has bright green fluorescence and can be detected at Ex/Em=496 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from cell components.A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
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    Discover our top product CASP3 ELISA Kit
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    FRET-based Assay Kit

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Protect Components A and B from light and from moisture.
    Lagerung
    -20 °C
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    Feinman, Deitch, Watkins, Abungu, Colorado, Kannan, Sheth, Caputo, Lu, Ramanathan, Attan, Badami, Doucet, Barlos, Bosch-Marce, Semenza, Xu: "HIF-1 mediates pathogenic inflammatory responses to intestinal ischemia-reperfusion injury." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 4, pp. G833-43, (2010) (PubMed).

    Reynolds, Stone, Mosley, Gendelman: "Nitrated {alpha}-synuclein-induced alterations in microglial immunity are regulated by CD4+ T cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 7, pp. 4137-49, (2009) (PubMed).

    Guo, Ran, Roberts, Zhou, Richardson, Sharan, Wu, Yang: "Suppression of atherogenesis by overexpression of glutathione peroxidase-4 in apolipoprotein E-deficient mice." in: Free radical biology & medicine, Vol. 44, Issue 3, pp. 343-52, (2008) (PubMed).

    Harris, Anderson, Lee: "The effect of proteasome inhibition on p53 degradation and proliferation in tonsil epithelial cells." in: Archives of otolaryngology--head & neck surgery, Vol. 134, Issue 2, pp. 157-63, (2008) (PubMed).

    Ireland, Leoni, Altaie, Birch, Coleman, Hunter-Lavin, Williams: "Measuring the secretion of heat shock proteins from cells." in: Methods (San Diego, Calif.), Vol. 43, Issue 3, pp. 176-83, (2007) (PubMed).

    James, Lee, Klingelhutz: "Human papillomavirus type 16 E6 activates NF-kappaB, induces cIAP-2 expression, and protects against apoptosis in a PDZ binding motif-dependent manner." in: Journal of virology, Vol. 80, Issue 11, pp. 5301-7, (2006) (PubMed).

  • Target
    Caspase 3 (CASP3)
    Andere Bezeichnung
    Caspase-3 (CASP3 Produkte)
    Synonyme
    CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
    Hintergrund
    Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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