Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PROCR ELISA Kit

PROCR Reaktivität: Human AA 18-210 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1672861
  • Target Alle PROCR ELISA Kits anzeigen
    PROCR (Protein C Receptor, Endothelial (PROCR))
    Bindungsspezifität
    AA 18-210
    Reaktivität
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312-20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human EPCR
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: S18-S210
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: S18-S210
    Featured
    Zu unserem meistverkauften PROCR ELISA Kit
    Top Product
    Discover our top product PROCR ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

    Plattentyp
    Pre-coated
    Protokoll
    human EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been precoated onto 96-well plates. Standards(NSO, S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCR amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human EPCR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCR standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 2.21, Standard deviation: 0.095, CV(%): 4.3
    • Sample 2: n=16, Mean(ng/ml): 8.45, Standard deviation: 0.313, CV(%): 3.7
    • Sample 3: n=16, Mean(ng/ml): 14.4, Standard deviation: 0.691, CV(%): 4.8,
    • Sample 1: n=24, Mean(ng/ml): 2.13, Standard deviation: 0.124, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 8.79, Standard deviation: 0.466, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 13.7, Standard deviation: 1.04, CV(%): 7.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle PROCR ELISA Kits anzeigen
    PROCR (Protein C Receptor, Endothelial (PROCR))
    Andere Bezeichnung
    PROCR (PROCR Produkte)
    Synonyme
    PROCR ELISA Kit, CCCA ELISA Kit, CCD41 ELISA Kit, EPCR ELISA Kit, AI325044 ELISA Kit, Ccca ELISA Kit, Ccd41 ELISA Kit, Epcr ELISA Kit, protein C receptor ELISA Kit, protein C receptor, endothelial ELISA Kit, PROCR ELISA Kit, Procr ELISA Kit
    Hintergrund

    Protein Function: Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex, plays a role in the protein C pathway controlling blood coagulation.

    Background: Endothelial protein C receptor(EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

    Synonyms: Endothelial protein C receptor,Activated protein C receptor,APC receptor,Endothelial cell protein C receptor,CD201,PROCR,EPCR,

    Full Gene Name: Endothelial protein C receptor

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gen-ID
    10544
    UniProt
    Q9UNN8
Sie sind hier:
Kundenservice