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PTX3 ELISA Kit

PTX3 Reaktivität: Human AA 18-381 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Saliva, Serum
Produktnummer ABIN1672819
  • Target Alle PTX3 ELISA Kits anzeigen
    PTX3 (Pentraxin 3 (PTX3))
    Bindungsspezifität
    AA 18-381
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312-20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PTX3/Pentraxin 3
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (EDTA), Saliva
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: E18-S381
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E18-S381
    Featured
    Zu unserem meistverkauften PTX3 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    human PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PTX3 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PTX3 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.22, CV(%): 6.3
    • Sample 2: n=16, Mean(ng/ml): 7.8, Standard deviation: 0.45, CV(%): 5.8
    • Sample 3: n=16, Mean(ng/ml): 15.7, Standard deviation: 1.0, CV(%): 6.4,
    • Sample 1: n=24, Mean(ng/ml): 3.86, Standard deviation: 0.28, CV(%): 7.3
    • Sample 2: n=24, Mean(ng/ml): 8.1, Standard deviation: 0.48, CV(%): 5.9
    • Sample 3: n=24, Mean(ng/ml): 15.3, Standard deviation: 1.16, CV(%): 7.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Bilgin, Haliloglu, Yaman, Ay, Bilgili, Arslantas, Ture Ozdemir, Haklar, Cinel, Mulazimoglu: "Sequential Measurements of Pentraxin 3 Serum Levels in Patients with Ventilator-Associated Pneumonia: A Nested Case-Control Study." in: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, Vol. 2018, pp. 4074169, (2018) (PubMed).

    Sağ, Sağ, Tekeoğlu, Kamanli, Nas, Harman: "The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis." in: Archives of rheumatology, Vol. 33, Issue 1, pp. 39-44, (2018) (PubMed).

    Kim, Won, Lee, Chun, Yoon, Kim, Kim: "Pentraxin 3 as a clinical marker in children with lower respiratory tract infection." in: Pediatric pulmonology, Vol. 51, Issue 1, pp. 42-8, (2016) (PubMed).

    Patel, Shanthraj, Bhagyalakshmi, Garg, Vallakati: "Comparative evaluation of pentraxin 3 levels in GCF during canine retraction with active tieback and NiTi coil spring: An in vivo study." in: Journal of orthodontic science, Vol. 5, Issue 2, pp. 52-6, (2016) (PubMed).

    Deveer, Deveer, Basaran, Turkcu, Akbaba, Cullu, Turhan, Kucuk, Kasap: "Serum Copeptin, Pentraxin 3, Anti-Mullerian Hormone Levels With Echocardiography and Carotid Artery Intima-Media Thickness in Adolescents With Polycystic Ovary Syndrome." in: Journal of clinical medicine research, Vol. 7, Issue 12, pp. 989-94, (2015) (PubMed).

  • Target Alle PTX3 ELISA Kits anzeigen
    PTX3 (Pentraxin 3 (PTX3))
    Andere Bezeichnung
    PTX3 (PTX3 Produkte)
    Synonyme
    fb05a06 ELISA Kit, wu:fb05a06 ELISA Kit, TNFAIP5 ELISA Kit, TSG-14 ELISA Kit, PPTX ELISA Kit, AI607804 ELISA Kit, si:ch211-272f3.3 ELISA Kit, pentraxin 3 ELISA Kit, pentraxin related gene ELISA Kit, si:ch211-272f3.3 ELISA Kit, LOC100230343 ELISA Kit, PTX3 ELISA Kit, Ptx3 ELISA Kit
    Hintergrund

    Protein Function: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

    Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

    Synonyms: Pentraxin-related protein PTX3,Pentaxin-related protein PTX3,Tumor necrosis factor alpha-induced protein 5,TNF alpha-induced protein 5,Tumor necrosis factor-inducible gene 14 protein,TSG-14,PTX3,TNFAIP5, TSG14,

    Full Gene Name: Pentraxin-related protein PTX3

    Cellular Localisation: Secreted.
    Gen-ID
    5806
    UniProt
    P26022
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