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Peroxiredoxin 1 ELISA Kit

PRDX1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN1572000
  • Target Alle Peroxiredoxin 1 (PRDX1) ELISA Kits anzeigen
    Peroxiredoxin 1 (PRDX1)
    Reaktivität
    • 3
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PRDX1 in mouse serum, plasma, tissue homogenates and other biological fluids.
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of this index.
    Kreuzreaktivität (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensitivität
    0.103 ng/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Testdurchführung

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Testpräzision
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Haltbarkeit
    12 months
  • Target Alle Peroxiredoxin 1 (PRDX1) ELISA Kits anzeigen
    Peroxiredoxin 1 (PRDX1)
    Andere Bezeichnung
    PRDX1 (PRDX1 Produkte)
    Synonyme
    MSP23 ELISA Kit, NKEF-A ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, PRX1 ELISA Kit, PRXI ELISA Kit, TDPX2 ELISA Kit, NkefA ELISA Kit, OSF-3 ELISA Kit, OSF3 ELISA Kit, Paga ELISA Kit, PrdxI ELISA Kit, PrxI ELISA Kit, TDX2 ELISA Kit, TPxA ELISA Kit, Tdpx2 ELISA Kit, prx1 ELISA Kit, Hbp23 ELISA Kit, MGC80194 ELISA Kit, MGC84820 ELISA Kit, PRDX1 ELISA Kit, msp23 ELISA Kit, nkefa ELISA Kit, pag ELISA Kit, paga ELISA Kit, pagb ELISA Kit, prx-1 ELISA Kit, prxi ELISA Kit, tdpx2 ELISA Kit, hm:zehl0637 ELISA Kit, zgc:110343 ELISA Kit, TPX-2 ELISA Kit, peroxiredoxin 1 ELISA Kit, peroxiredoxin 1 S homeolog ELISA Kit, peroxiredoxin-1 pseudogene ELISA Kit, PRDX1 ELISA Kit, Prdx1 ELISA Kit, prdx1.S ELISA Kit, prdx1 ELISA Kit, LOC100350232 ELISA Kit
    Hintergrund
    Alternative name: MSP23, NKEFA, PAG, PAGA, PAGB, TDPX2, Natural killer cell-enhancing factor A, Proliferation-associated, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2
    Gen-ID
    18477
    UniProt
    P35700
    Pathways
    p53 Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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