Annexin V-FITC Apoptosis Detection Kit

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Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin A5 L homeolog
  • ANXA5
  • anxa5b
  • anxa5
  • Anxa5
  • anxa5.L
Alternativen
Human Annexin V ELISA Kit
Konjugat
FITC
Applikation
Flow Cytometry (FACS)
Optionen
Hersteller
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Verwendungszweck The assay combines annexin V staining of PS membrane events with the staining of cell nucleus with PI or AAD-7 in living cells to distinguish from dead cells.
Nachweismethode Fluorometric
Produktmerkmale Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to PS is Ca2+ dependent). Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy
Andere Bezeichnung Annexin V (ANXA5 ELISA Kit Abstract)
Hintergrund Annexin A5 (or Annexin V) is a cellular protein in the annexin group. Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death.
Forschungsgebiet Apoptosis
Plattentyp Strips (12 x 8)
Testdurchführung

Staining Protocol:
1.Dilute the Binding buffer (10×) to work solution (1×) with distilled water.
2.Harvest cell, then wash once with cold PBS, remove the PBS from the cell pellet.
3.Wash again with cold 1× Binding buffer, remove the Binding buffer from the cell pellet.
4.Resuspend cells in cold 1× Binding buffer to a concentration of 1×10 6 cells/mL.
5.Add 100µL of cells (1×10 5 ) to each appropriate tube.
6.Add 5µL of Annexin V-FITC to appropriate tubes.
7.Gently vortex each tube and incubate for 10 minutes at room temperature in dark.
8.Add 5µL of PI solution and incubate for 5 min at room temperature in dark.
9.Wash cells once in PBS and resuspend in PBS.
10.Analyze by flow cytometry within 4 hours.

Beschränkungen Nur für Forschungszwecke einsetzbar
Konservierungsmittel Sodium azide, Thimerosal (Merthiolate)