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Prolactin ELISA Kit

PRL Reaktivität: Ratte, Maus Colorimetric Sandwich ELISA
Produktnummer ABIN1326881
  • Target Alle Prolactin (PRL) ELISA Kits anzeigen
    Prolactin (PRL)
    Reaktivität
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    The prolactin kit is based on a solid phase sandwich ELISA method. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Diluted horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin.
    Analytische Methode
    Quantitative
    Featured
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  • Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Clarke, Russell, Findlay, Sastra, Anderson, Skinner, Atkins, Zajac, Davey: "A ROLE FOR THE CALCITONIN RECEPTOR TO LIMIT BONE LOSS DURING LACTATION IN FEMALE MICE BY INHIBITING OSTEOCYTIC OSTEOLYSIS." in: Endocrinology, pp. en20151345, (2015) (PubMed).

    Kahraman, Dirice, De Jesus, Hu, Kulkarni: "Maternal insulin resistance and transient hyperglycemia impact the metabolic and endocrine phenotypes of offspring." in: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 10, pp. E906-18, (2014) (PubMed).

  • Target Alle Prolactin (PRL) ELISA Kits anzeigen
    Prolactin (PRL)
    Andere Bezeichnung
    Prolactin (PRL Produkte)
    Synonyme
    PRL ELISA Kit, prolactin ELISA Kit, PRLB ELISA Kit, PRLSD1 ELISA Kit, Prl1a1 ELISA Kit, Prol ELISA Kit, RATPRLSD1 ELISA Kit, RNPROL ELISA Kit, AV290867 ELISA Kit, prl ELISA Kit, prolactin ELISA Kit, prolactin, gene 2 L homeolog ELISA Kit, PROLACTIN protein ELISA Kit, prolactin-like ELISA Kit, PRL ELISA Kit, prl.2.L ELISA Kit, Prl ELISA Kit, LOC100136792 ELISA Kit, LOC100136580 ELISA Kit, PROLACTIN ELISA Kit, LOC101843376 ELISA Kit
    Hintergrund
    Prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland. Plasma and pituitary PRL levels are significantly greater in adult female than in male rats. This difference is thought to be brought about by ovarian steroids. A similar sex difference was found in pituitary PRL content in normal mice from 30 days of age. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications.
    Pathways
    JAK-STAT Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
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