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IgG ELISA Kit

Reaktivität: Rind (Kuh) Heavy & Light Chain Colorimetric Sandwich ELISA 10-2000 ng/mL Cell Culture Supernatant, Serum
Produktnummer ABIN1113190
  • Target Alle IgG ELISA Kits anzeigen
    IgG
    Bindungsspezifität
    Heavy & Light Chain
    Reaktivität
    • 9
    • 9
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Rind (Kuh)
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    10-2000 ng/mL
    Untere Nachweisgrenze
    10 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera
    Marke
    FastELISA®
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    The method enables the detection of bovine IgG.
    Produktmerkmale
    The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.
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  • Kommentare

    One Step ELISA 45 min total time
    IgG contaminations of about 20 ppm

    Testdauer
    < 1 h
    Plattentyp
    Pre-coated
    Protokoll
    A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    Aufbereitung der Reagenzien

    dilution

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle IgG ELISA Kits anzeigen
    IgG
    Andere Bezeichnung
    Immunoglobulin G (IgG Produkte)
    Substanzklasse
    Antibody
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