Maus PTPN11 ELISA Kit

Recommended PTPN11 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) ELISA Kits
  • BPTP3
  • CFC
  • NS1
  • PTP-1D
  • PTP2C
  • SH-PTP2
  • SH-PTP3
  • SHP2
  • 2700084A17Rik
  • AW536184
  • PTP1D
  • SAP-2
  • SHP-2
  • Shp2
  • Syp
  • SYP
  • protein tyrosine phosphatase, non-receptor type 11
  • PTPN11
  • Ptpn11
Epitop
pTyr542
Reaktivität
Human, Maus, Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
14
14
10
1
1
1
1
1
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Produktnummer ABIN2747886
$ 578.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
1 ABIN2747888 Cell ELISA Cell Culture Cells Anmelden zum Anzeigen
1 ABIN2747887 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN1380083 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380082 Cell ELISA Anmelden zum Anzeigen
1 ABIN1381495 Cell ELISA Anmelden zum Anzeigen
1 ABIN512069 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Anmelden zum Anzeigen
1 ABIN2114817 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114818 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114816 Cell ELISA Anmelden zum Anzeigen
1 ABIN2685332 Cell ELISA Cell Samples, Suspension Cell Culture Anmelden zum Anzeigen

General

Antigen Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) ELISA Kits
Epitop pTyr542
Reaktivität Human, Maus, Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(14), (14), (10), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails PTPN11 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Human/Mouse/Rat Phospho-SHP2 (Y542) ELISA Kit. This assay semi-quantitatively measures phophorylated SHP2 (Tyr542) in lysate samples.
Proben Cell Lysate, Tissue Lysate
Nachweismethode Colorimetric
Analytische Methode Semi-Quantitative
Spezifität The antibody pair provided in this kit recognizes human, mouse, and rat SHP2 phosphorylated at site Tyrosine-542
Produktmerkmale
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Benötigtes Material
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Antigendetails

Produktdetails PTPN11 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung SHP2 (PTPN11 ELISA Kit Abstract)
Hintergrund SHP2 phosphorylated at Tyrosine-542
Gen-ID 5781
UniProt Q06124
Forschungsgebiet Neurology
Pathways JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails PTPN11 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Testdurchführung Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails PTPN11 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit 6 months