anti-Human PTPN11 Antikörper für Intracellular Staining

Recommended PTPN11 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) Antikörper
  • BPTP3
  • CFC
  • NS1
  • PTP-1D
  • PTP2C
  • SH-PTP2
  • SH-PTP3
  • SHP2
  • 2700084A17Rik
  • AW536184
  • PTP1D
  • SAP-2
  • SHP-2
  • Shp2
  • Syp
  • SYP
  • protein tyrosine phosphatase, non-receptor type 11
  • PTPN11
  • Ptpn11
Epitop
pTyr542
54
46
25
21
19
17
15
15
15
10
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Ratte (Rattus)
367
231
201
14
13
13
11
11
10
9
7
4
3
3
2
2
2
1
Wirt
Maus
298
57
19
2
1
Klonalität
Monoklonal
Konjugat
Dieser PTPN11 Antikörper ist konjugiert mit PE
9
9
9
6
5
5
4
4
4
4
4
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Intracellular Staining (ICS), Western Blotting (WB)
290
126
106
85
52
49
33
22
18
6
3
3
3
1
1
1
1
Optionen
Hersteller
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Produktnummer ABIN1177171
247,50 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1177172 ICS WB Alexa Fluor 647 Mouse IgG1, kappa pTyr542 Anmelden zum Anzeigen L99-921 5

anti-PTPN11 Antikörper für Intracellular Staining mit den meisten Publikationen

Ähnliche anti-PTPN11 Antikörper

Applikation / Reaktivität Ratte (Rattus) Human
ELISA 38 Antikörper 104 Antikörper
Enzyme Immunoassay (EIA) 2 Antikörper 3 Antikörper
Immunocytochemistry (ICC) 2 Antikörper
Immunofluorescence (IF) 17 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 1 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antikörper
Immunohistochemistry (IHC) 71 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antikörper
Immunoprecipitation (IP) 10 Antikörper
Intracellular Staining (ICS) 2 Antikörper
Neutralization (Neut) 1 Antikörper
Western Blotting (WB) 139 Antikörper
Cytometry by Time of Flight (CyTOF) 1 Antikörper
Dot Blot (DB) 3 Antikörper
Flow Cytometry (FACS) 33 Antikörper

General

Antigen Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) Antikörper
Epitop pTyr542
(54), (46), (25), (21), (19), (17), (15), (15), (15), (10), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Ratte (Rattus)
(367), (231), (201), (14), (13), (13), (11), (11), (10), (9), (7), (4), (3), (3), (2), (2), (2), (1)
Wirt Maus
(298), (57), (19), (2), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser PTPN11 Antikörper ist konjugiert mit PE
(9), (9), (9), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Intracellular Staining (ICS), Western Blotting (WB)
(290), (126), (106), (85), (52), (49), (33), (22), (18), (6), (3), (3), (3), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-PTPN11 Antikörper

Target Details PTPN11 Anwendungsinformationen Handhabung ProductDetails: References for anti-PTPN11 antibody (ABIN1177171) Bilder
Marke BD Phosflow™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human SHP2 Peptide
Klon L99-921
Isotyp IgG1, kappa

Target Details PTPN11

Produktdetails anti-PTPN11 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-PTPN11 antibody (ABIN1177171) Bilder zurück nach oben
Antigen
Andere Bezeichnung SHP2 (PTPN11 Antibody Abstract)
Hintergrund SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 reportedly contains two SH2 domains, both of which are N-terminal to the PTP catalytic domain. SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580. Phosphorylation of Y542 creates an interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an interaction at the C-terminal that stimulates PTP activity. The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2). The homologous phosphorylation site in the PTP2Ci splice variant (isoform 1) is Y546. The specificity of this antibody was validated by confirming, using western blot analysis, that RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542).
Synonyms: PTN11, PTP1D, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHP-2, SHPTP2
Forschungsgebiet Neurology
Pathways JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-PTPN11 Antikörper Target Details PTPN11 Handhabung ProductDetails: References for anti-PTPN11 antibody (ABIN1177171) Bilder zurück nach oben
Applikationshinweise This antibody conjugate is suitable for intracellular staining of cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PTPN11 Antikörper Target Details PTPN11 Anwendungsinformationen ProductDetails: References for anti-PTPN11 antibody (ABIN1177171) Bilder zurück nach oben
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

ProductDetails: References for anti-PTPN11 antibody (ABIN1177171)

Produktdetails anti-PTPN11 Antikörper Target Details PTPN11 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Fornasa, Groyer, Clement, Dimitrov, Compain, Gaston, Varthaman, Khallou-Laschet, Newman, Graff-Dubois, Nicoletti, Caligiuri: "TCR stimulation drives cleavage and shedding of the ITIM receptor CD31." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 10, pp. 5485-92, 2010

MacGillivray, Herrera-Abreu, Chow, Shek, Wang, Vachon, Feng, Siminovitch, McCulloch, Downey: "The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27190-8, 2003

Lu, Gong, Bar-Sagi, Cole: "Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling." in: Molecular cell, Vol. 8, Issue 4, pp. 759-69, 2001

Bennett, Tang, Sugimoto, Walsh, Neel: "Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 7335-9, 1994

Ahmad, Banville, Zhao, Fischer, Shen: "A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 6, pp. 2197-201, 1993