Maus Leptin ELISA Kit

Recommended Leptin Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Leptin (LEP) ELISA Kits
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
55
35
27
18
13
11
11
8
6
6
5
4
3
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
163.8-40000 pg/mL
Untere Nachweisgrenze
163.8 pg/mL
Applikation
ELISA
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2506965
423,24 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.70292 ABIN625156 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 4-1000 pg/mL 4 pg/mL Anmelden zum Anzeigen 19
9.70292 ABIN415517 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen 3
9.70292 ABIN365180 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen 5
9.70292 ABIN1029097 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen
9.70292 ABIN1115745 Sandwich ELISA Serum, Plasma, Biological Fluids 125-8000 pg/mL 125 pg/mL Anmelden zum Anzeigen 1
9.70292 ABIN612800 Sandwich ELISA Plasma, Cell Culture Supernatant 300 pg/mL Anmelden zum Anzeigen 7
9.70292 ABIN3198593 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Anmelden zum Anzeigen
9.70292 ABIN924822 Sandwich ELISA Cell Lysate, Serum, Plasma 20-1250 pg/mL 20 pg/mL Anmelden zum Anzeigen
9.70292 ABIN491793 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 41.2-30.000 pg/mL 41.2 pg/mL Anmelden zum Anzeigen
9.70292 ABIN455676 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen
9.70292 ABIN956062 Sandwich ELISA Anmelden zum Anzeigen
1 ABIN1995061 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN3032824 Competition ELISA Cell Culture Supernatant, Plasma, Serum 4.1-1000 pg/mL 4.1 pg/mL Anmelden zum Anzeigen
1 ABIN628075 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Anmelden zum Anzeigen
1 ABIN5592189 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen
1 ABIN5520826 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2114697 Cell Lysate, Serum, Plasma 20-1250 pg/mL 20 pg/mL Anmelden zum Anzeigen
1 ABIN1112737 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN5068253 Sandwich ELISA Plasma, Serum Anmelden zum Anzeigen
1 ABIN930284 Sandwich ELISA 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen

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General

Antigen Leptin (LEP) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(55), (35), (27), (18), (13), (11), (11), (8), (6), (6), (5), (4), (3), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 163.8-40000 pg/mL
Untere Nachweisgrenze 163.8 pg/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails Leptin ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of leptin in mouse serum, plasma, and other biological fluids not being tested
Marke EasyTest™
Proben Serum, Plasma
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of mouse leptin.
Sensitivität 49 pg/mL
Produktmerkmale EasyTest ELISA, a fast ELISA provides a simple and rapid quantitation of antigen in a range of sample matrices. Compared to standard ELISA, EasyTest ELISA requires only one wash, decreasing variations and significantly saves time. The whole process requires less than 2.5 hours and provides customers with high accuracy.
Bestandteile
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • mouse leptin Mix-4x 8 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x 8mL
  • Adhensive strip-2
  • Instruction manual-1.
Benötigtes Material 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Antigendetails

Produktdetails Leptin ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Leptin (LEP ELISA Kit Abstract)
Hintergrund Mouse Leptin is a protein product of the mouse obese gene. Mouse Leptin cDNA encodes a 167 aa residue protein with a 21 aa residue signal sequence that is cleaved to yield the 146 aa residue mature protein. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mature mouse Leptin shares approximately 96 % and 84 % amino acid (aa) sequence identity with the rat and human proteins, respectively.
Molekulargewicht 16 kDa
Gen-ID 16846
NCBI Accession NP_032519
UniProt P41160
Forschungsgebiet Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Anwendungsinformationen

Produktdetails Leptin ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Probenmenge 50 μL
Testdauer 2.5 h
Plattentyp Pre-coated
Protokoll This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse leptin has been pre-coated onto a microplate. Standards, samples and mouse leptin mix are pipetted into the wells and any Leptin present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of leptin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Aufbereitung der Reagenzien 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 100 ng/mL standard. Gently vortex to mix.Take 200 μL mouse leptin standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 40000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. mouse leptin Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1492 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Probennahme Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Aufbereitung der Proben Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:10.
Testdurchführung 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Mouse leptin Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 2 hours at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Ergebnisberechnung 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Testpräzision intra CV<10%, inter CV <15%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Leptin ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Buffer 0.05 % Proclin 300
Konservierungsmittel ProClin
Vorsichtsmaßnahmen The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Lagerung 4 °C/-20 °C

Bilder

Produktdetails Leptin ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Leptin ELISA Kit (LEP) (ABIN2506965) Leptin (LEP) ELISA Kit