Human IL12A ELISA Kit

Recommended IL12A Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Interleukin 12 alpha (IL12A) ELISA Kits
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • Il12a
  • IL12A
Epitop
AA 23-328, AA 23-219
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
12
8
7
6
5
5
3
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
7.8-500 pg/mL
Untere Nachweisgrenze
7.8 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN411309
350,95 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.702543 ABIN414875 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Anmelden zum Anzeigen
9.702543 ABIN1570602 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
9.702543 ABIN1055170 Sandwich ELISA Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma 50-1000 pg/mL 50 pg/mL Anmelden zum Anzeigen
1 ABIN841917 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN455529 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN5595253 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN4993521 Sandwich ELISA Plasma, Serum, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Anmelden zum Anzeigen
1 ABIN5525609 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN5596358 Sandwich ELISA Anmelden zum Anzeigen
1 ABIN4969961 Sandwich ELISA 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN5074426 Anmelden zum Anzeigen
1 ABIN5074425 Anmelden zum Anzeigen

IL12A ELISA Kit für ELISA mit den meisten Publikationen

  • Human IL12A ELISA Kit für Sandwich ELISA - ABIN411309 (9 Publications): Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock. in The Journal of biological chemistry 2011 (PubMed)

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General

Antigen Interleukin 12 alpha (IL12A) ELISA Kits
Epitop AA 23-328, AA 23-219
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(12), (8), (7), (6), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 7.8-500 pg/mL
Untere Nachweisgrenze 7.8 pg/mL
Applikation ELISA
Pubmed 9 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails IL12A ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411309) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-12(p70)
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: sf21
Immunogen sequence: (I23-S328)+(R23-S219)
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <2pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: (I23-S328)+(R23-S219)

Antigendetails

Produktdetails IL12A ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411309) Bilder zurück nach oben
Antigen
Andere Bezeichnung IL12A (IL12A ELISA Kit Abstract)
Hintergrund

Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Synonyms: Interleukin 12 ,Interleukin 12, P35 ,Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ,Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ,IL12A ,hCG_1685939 ,

Full Gene Name: interleukin 12A

Gen-ID 3592
UniProt O60595
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails IL12A ELISA Kit Antigendetails Handhabung ProductDetails: References for IL12A Kit (ABIN411309) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll human IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-12(P70) has been precoated onto 96-well plates. Standards(sf21, (I23-S328)+(R23-S219)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-12(P70) amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-12(P70) standard solution and each sample is measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 44, Standard deviation: 2.16, CV(%): 4.9
  • Sample 2: n=16, Mean(pg/ml): 127, Standard deviation: 7.24, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 283, Standard deviation: 17.83, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 67, Standard deviation: 3.89, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 158, Standard deviation: 9.48, CV(%): 6
  • Sample 3: n=24, Mean(pg/ml): 322, Standard deviation: 23.2, CV(%): 7.2
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for IL12A Kit (ABIN411309) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for IL12A Kit (ABIN411309)

Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011

Li, Yu, Ye, Wang, Yang: "Lactobacillus S-layer protein inhibition of Salmonella-induced reorganization of the cytoskeleton and activation of MAPK signalling pathways in Caco-2 cells." in: Microbiology (Reading, England), Vol. 157, Issue Pt 9, pp. 2639-46, 2011

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Produktdetails IL12A ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL12A Kit (ABIN411309) zurück nach oben
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ELISA image for IL12A ELISA Kit (Interleukin 12 alpha) (ABIN411309) Human IL-12(p70) PicoKine ELISA Kit standard curve