Human IL-3 ELISA Kit

Recommended IL-3 Product (geliefert von: Anmelden zum Anzeigen )

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Interleukin 3 (IL-3) ELISA Kits
  • BPA
  • Csfmu
  • HCGF
  • Il-3
  • MCGF
  • PSF
  • IL-3
  • MULTI-CSF
  • interleukin 3
  • interleukin 3 (colony-stimulating factor, multiple)
  • Il3
  • IL3
Epitop
AA 20-152
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
34
26
17
7
6
5
3
3
2
2
2
2
2
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6-1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN411297
424,29 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.70292 ABIN414883 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
9.70292 ABIN365167 Sandwich ELISA Serum, Plasma 3.12-200 pg/mL 3.12 pg/mL Anmelden zum Anzeigen
9.70292 ABIN625325 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 20-12000 pg/mL 20 pg/mL Anmelden zum Anzeigen
9.70292 ABIN3198578 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
9.70292 ABIN924780 Sandwich ELISA Cell Lysate, Serum, Plasma 16-4000 pg/mL 16 pg/mL Anmelden zum Anzeigen
9.70292 ABIN1115660 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2708285 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
9.70292 ABIN5026937 Sandwich ELISA Plasma, Serum, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2114316 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Anmelden zum Anzeigen
9.70292 ABIN2064521 Cell Culture Supernatant, Serum, Plasma Anmelden zum Anzeigen
1 ABIN1029000 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN3032948 Competition ELISA Cell Culture Supernatant, Plasma, Serum 16.5-12000 pg/mL 16.5 pg/mL Anmelden zum Anzeigen
1 ABIN626508 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Anmelden zum Anzeigen
1 ABIN5595248 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN2684686 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen 3
1 ABIN1112646 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN5525601 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2748861 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.3-2000 pg/mL 31.3 pg/mL Anmelden zum Anzeigen
1 ABIN3045587 Sandwich ELISA Plasma, Biological Fluids, Serum, Tissue Samples 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN2114315 Cell Lysate, Serum, Plasma 16-4000 pg/mL 16 pg/mL Anmelden zum Anzeigen

IL-3 ELISA Kit für ELISA mit den meisten Publikationen

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General

Antigen Interleukin 3 (IL-3) ELISA Kits
Epitop AA 20-152
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(34), (26), (17), (7), (6), (5), (3), (3), (2), (2), (2), (2), (2)
Methodentyp Sandwich ELISA
Detektionsbereich 15.6-1000 pg/mL
Untere Nachweisgrenze 15.6 pg/mL
Applikation ELISA
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails IL-3 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL-3 Kit (ABIN411297) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-3
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli,A20-F152
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <1pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A20-F152

Antigendetails

Produktdetails IL-3 ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for IL-3 Kit (ABIN411297) Bilder zurück nach oben
Antigen
Andere Bezeichnung IL3 (IL-3 ELISA Kit Abstract)
Hintergrund

Protein Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Synonyms: Interleukin-3,IL-3,Hematopoietic growth factor,Mast cell growth factor,MCGF,Multipotential colony-stimulating factor,P-cell-stimulating factor,IL3,

Full Gene Name: Interleukin-3

Cellular Localisation: Secreted.
Gen-ID 3562
UniProt P08700
Forschungsgebiet Immunology, Innate Immunity, Cytokines, Virology, Inflammation, Cancer
Pathways JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie

Anwendungsinformationen

Produktdetails IL-3 ELISA Kit Antigendetails Handhabung ProductDetails: References for IL-3 Kit (ABIN411297) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the IL-3 family.

Tissue Specificity: Activated T-cells, mast cells, natural killer cells.

Plattentyp Pre-coated
Protokoll human IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-3 has been precoated onto 96-well plates. Standards (E.coli,A20-F152) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-3 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-3 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 4.56, CV(%): 4.8
  • Sample 2: n=16, Mean(pg/ml): 323, Standard deviation: 12.6, CV(%): 3.9
  • Sample 3: n=16, Mean(pg/ml): 590, Standard deviation: 21.83, CV(%): 3.7,
  • Sample 1: n=24, Mean(pg/ml): 108, Standard deviation: 8.75, CV(%): 8.1
  • Sample 2: n=24, Mean(pg/ml): 372, Standard deviation: 24.18, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 625, Standard deviation: 33.75, CV(%): 5.4
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails IL-3 ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for IL-3 Kit (ABIN411297) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for IL-3 Kit (ABIN411297)

Produktdetails IL-3 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, 2014

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, 2012

Allgemeine Veröffentlichungen

Yang, Kovacic, Kriz, Wolf, Clark, Wellems, Nienhuis, Epstein: "The human genes for GM-CSF and IL 3 are closely linked in tandem on chromosome 5." in: Blood, Vol. 71, Issue 4, pp. 958-61, 1988

Clark-Lewis, Aebersold, Ziltener, Schrader, Hood, Kent: "Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3." in: Science (New York, N.Y.), Vol. 231, Issue 4734, pp. 134-9, 1986

Fung, Hapel, Ymer, Cohen, Johnson, Campbell, Young: "Molecular cloning of cDNA for murine interleukin-3." in: Nature, Vol. 307, Issue 5948, pp. 233-7, 1984

Bilder

Produktdetails IL-3 ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IL-3 Kit (ABIN411297) zurück nach oben
Bilder des Herstellers
ELISA image for IL-3 ELISA Kit (Interleukin 3) (ABIN411297) Human IL-3 PicoKine ELISA Kit standard curve