anti-Human CSF2RA Antikörper für Flow Cytometry

Recommended CSF2RA Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) Antikörper
  • CD116
  • CDw116
  • CSF2R
  • CSF2RAX
  • CSF2RAY
  • CSF2RX
  • CSF2RY
  • GM-CSF-R-alpha
  • GMCSFR
  • GMR
  • SMDP4
  • Csfgmra
  • GM-CSF-Ra
  • GM-CSFR
  • colony stimulating factor 2 receptor, alpha
  • colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
  • CSF2RA
  • Csf2ra
Reaktivität
Human
163
34
8
Wirt
Maus
89
81
16
2
Klonalität
Monoklonal
Konjugat
Dieser CSF2RA Antikörper ist konjugiert mit FITC
16
11
10
6
6
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS)
105
103
49
26
15
8
4
3
3
3
2
2
2
2
2
2
1
1
Hersteller
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Bilder

Produktnummer ABIN487471
374,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.342041 ABIN1997316 FACS PE Mouse IgG1 AA 1-320 Anmelden zum Anzeigen 03
13.342041 ABIN2662955 FACS ICFC PE Mouse IgG1 kappa Anmelden zum Anzeigen 4H1 4
13.342041 ABIN2661473 Func ICC FACS IF ICFC IP IHC WB FITC Mouse IgG1 kappa Anmelden zum Anzeigen 4H1 5
13.342041 ABIN4897901 FACS PE Mouse IgG1 Extracellular Domain Anmelden zum Anzeigen 31916
13.342041 ABIN4897900 FACS Allophycocyanin Mouse IgG1 Extracellular Domain Anmelden zum Anzeigen 31916
13.342041 ABIN2213632 FACS FITC Rabbit IgG AA 1-320 Anmelden zum Anzeigen 009
13.342041 ABIN2213634 FACS PE Rabbit IgG AA 1-320 Anmelden zum Anzeigen 009
13.342041 ABIN1981895 IHC (f) FACS IP WB PE Mouse IgG1 Anmelden zum Anzeigen 4H1 3
13.342041 ABIN5506606 FACS APC Rabbit IgG AA 1-320 Anmelden zum Anzeigen 009
13.342041 ABIN2681827 ELISA FACS Rabbit IgG AA 1-320 Anmelden zum Anzeigen 009
13.342041 ABIN514673 FACS WB Mouse AA 1-400, full length Anmelden zum Anzeigen Polyclonal
13.342041 ABIN5506607 FACS Mouse IgG1 AA 1-320 Anmelden zum Anzeigen 03
13.342041 ABIN514674 FACS WB Mouse AA 1-400, full length Anmelden zum Anzeigen Polyclonal
1 ABIN205440 FACS IHC (fro) IP WB Mouse IgG1, kappa Anmelden zum Anzeigen 4H1
1 ABIN475306 FACS PE Mouse IgG1, kappa Anmelden zum Anzeigen 4H1
1 ABIN473799 FACS FITC Mouse IgG1, kappa Anmelden zum Anzeigen 4H1
1 ABIN1981902 IHC (f) FACS IP WB Mouse IgG1 Anmelden zum Anzeigen 4H1 3
1 ABIN487470 FACS Mouse IgG1 Anmelden zum Anzeigen 4H1
1 ABIN475158 FACS Biotin Mouse IgG1, kappa Anmelden zum Anzeigen 4H1
1 ABIN4315185 FACS Alexa Fluor 594 Mouse IgG1 Anmelden zum Anzeigen 31916

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General

Antigen Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) Antikörper
Reaktivität Human
(163), (34), (8)
Wirt Maus
(89), (81), (16), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CSF2RA Antikörper ist konjugiert mit FITC
(16), (11), (10), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Flow Cytometry (FACS)
(105), (103), (49), (26), (15), (8), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CSF2RA Antikörper

Target Details CSF2RA Anwendungsinformationen Handhabung Bilder
Spezifität This antibody reacts with Human CD116 antigen (GM-CSF receptor alpha-chain).
Kreuzreaktivität (Details) Species reactivity (tested):Human.
Produktmerkmale Synonyms: GM-CSF-R-alpha, GMR, CSF2R, CSF2RY, GMCSF Receptor alpha, GM-CSF Receptor alpha,Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Reinigung Protein-A Sepharose Chromatography.
Immunogen COS cells overexpressing GM-CSF receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte.
Klon 4H1
Isotyp IgG1

Target Details CSF2RA

Produktdetails anti-CSF2RA Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung CD116 / CSF2RA (CSF2RA Antibody Abstract)
Hintergrund CD116/GM-CSFRα/CSF2RA is the alpha subunit of the heterodimeric cytokine receptor for colony stimulating factor 2/GM-CSF, a cytokine which controls the proliferation, differentiation, and function of granulocytes and macrophages. CD116 functions as a low affinity receptor that forms a high affinity receptor when complexed with CDw131, the Synonyms: CSF2R, CSF2RY, GM-CSF Receptor alpha, GM-CSF-R-alpha, GMCSF Receptor alpha, GMR, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Gen-ID 1438
UniProt P15509
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Handhabung Bilder zurück nach oben
Applikationshinweise Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Transfectant cells. Detailed procedure is provided in the following Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll Flow Cytometric Aanalysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD116 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD116 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood monocytes
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Handhabung

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.05 mg/mL
Buffer PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Haltbarkeit 12 months

Bilder

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CSF2RA Antikörper (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage))  (FITC) (ABIN487471) anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) antibody (FITC)