Ratte (Rattus) Angiotensin II ELISA Kit

Recommended Angiotensin II Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Angiotensin II (Ang II) ELISA Kits
  • ANHU
  • SERPINA8
  • ANRT
  • Ang
  • AngII
  • PAT
  • AI265500
  • AngI
  • Aogen
  • Serpina8
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • AGT
  • Agt
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
21
17
15
10
9
8
7
6
5
2
2
1
1
Methodentyp
Competition ELISA
Detektionsbereich
24.69-2000 pg/mL
Untere Nachweisgrenze
24.69 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN416068
$ 576.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.541964 ABIN1558956 Competition ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2801458 Competition ELISA Serum, Plasma 0.49-2000 pg/mL 0.49 pg/mL Anmelden zum Anzeigen
1 ABIN368044 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate 4.7-300 pg/mL 4.7 pg/mL Anmelden zum Anzeigen 5
1 ABIN1568349 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN5651797 Competition ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 24.69 pg/mL - 2000 pg/mL 24.69 pg/mL Anmelden zum Anzeigen
1 ABIN5525882 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN2056122 Competition ELISA 100-2500 pg/mL 100 pg/mL Anmelden zum Anzeigen
1 ABIN956379 Competition ELISA 24.69-2000 pg/mL 24.69 pg/mL Anmelden zum Anzeigen
1 ABIN5068179 Competition ELISA Plasma, Serum Anmelden zum Anzeigen
1 ABIN2871308 Competition ELISA 0.08-1.55 ng/mL 0.08 ng/mL Anmelden zum Anzeigen
1 ABIN2541380 Serum, Plasma, Tissue Homogenate, Biological Fluids 7-400 pg/mL 7 pg/mL Anmelden zum Anzeigen
1 ABIN2541355 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 24.69-2000 pg/mL 24.69 pg/mL Anmelden zum Anzeigen
1 ABIN2871085 Competition ELISA Anmelden zum Anzeigen
1 ABIN2871086 Competition ELISA Anmelden zum Anzeigen
1 ABIN4967134 Competition ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2871307 Competition ELISA Anmelden zum Anzeigen

General

Antigen Angiotensin II (Ang II) ELISA Kits
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(21), (17), (15), (10), (9), (8), (7), (6), (5), (2), (2), (1), (1)
Methodentyp Competition ELISA
Detektionsbereich 24.69-2000 pg/mL
Untere Nachweisgrenze 24.69 pg/mL
Applikation ELISA
Pubmed 18 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails Angiotensin II ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN416068) Bilder
Verwendungszweck The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids
Proben Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität

This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Kreuzreaktivität (Details) No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.
Sensitivität 9.34 pg/mL
Bestandteile
  • Pre-coated, ready to use 96-well strip plate
  • Plate sealer for 96 wells
  • Standard Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Stop Solution
  • Standard
  • Detection Reagent A
  • Detection Reagent B
  • TMB Substrate
  • Wash Buffer (30 × concentrate)
  • Instruction manual
Benötigtes Material
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Antigendetails

Produktdetails Angiotensin II ELISA Kit Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN416068) Bilder zurück nach oben
Antigen
Andere Bezeichnung AngII (Ang II ELISA Kit Abstract)
Pathways JAK-STAT Signalweg, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity

Anwendungsinformationen

Produktdetails Angiotensin II ELISA Kit Antigendetails Handhabung Referenzen für Angiotensin II Kit (ABIN416068) Bilder zurück nach oben
Applikationshinweise
  • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
  • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
  • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
  • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
  • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
  • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
  • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
  • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
  • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
  • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
Kommentare

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge 50 μL
Testdauer 2 h
Plattentyp Pre-coated
Protokoll This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin II (AngII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated antibody specific to Angiotensin II (AngII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin II (AngII) in the sample.
Aufbereitung der Reagenzien
  • Bring all kit components and samples to room temperature (18-25°C) before use.
  • Standard - Reconstitute the Standard with 0.5mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 2,000pg/mL. Please prepare 5 tubes containing 0.6mL Standard Diluent and produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
  • Assay Diluent A and Assay Diluent B - Dilute 6mL of Assay Diluent A or B Concentrate(2×) with 6mL of deionized or distilled water to prepare 12mL of Assay Diluent A or B. (In fact, more than 6mL Assay Diluent A and Assay Diluent B are contained in the bottles. Therefore, in every test, please precisely pipette required amount of Diluent and make double dilution in a new container. The prepared working dilution cannot be frozen.)
  • Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with working Assay Diluent A or B, respectively (1:100).
  • Wash Solution - Dilute 20mL of Wash Solution concentrate (30×) with 580mL of deionized or distilled water to prepare 600mL of Wash Solution (1×).
  • TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.
Note:
  • Making serial dilution in the wells directly is not permitted.
  • Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37°C directly.
  • Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
  • Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL for once pipetting.
  • The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
  • If crystals have formed in the Wash Solution concentrate (30×), warm to room temperature and mix gently until the crystals are completely dissolved.
  • Contaminated water or container for reagent preparation will influence the detection result.
Probennahme Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C

Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.

Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.

Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Aufbereitung der Proben
  • We are only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. Sample should be diluted by 0.01mol/L PBS(PH=7.0-7.2).
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.
  • Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g.antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
  • Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.
  • Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Testdurchführung
  1. Prepare all reagents, samples and standards,
  2. Add 50μL standard or sample to each well.
    And then add 50μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37 °C,
  3. Aspirate and wash 3 times,
  4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  5. Aspirate and wash 5 times,
  6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  7. Add 50μL Stop Solution. Read at 450 nm immediately.
Ergebnisberechnung This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between AngII concentration in the sample and the assay signal intensity. Average the duplicate readings for each standard, control, and samples. Create a standard curve on log-log or semi-log graph paper, with the log of AngII concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
Testpräzision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Referenzen für Angiotensin II Kit (ABIN416068) Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handhabung

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung 4 °C
Informationen zur Lagerung
  • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
  • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
    Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
  • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
Haltbarkeit 6 months

Referenzen für Angiotensin II Kit (ABIN416068)

Produktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Wang, Qian, Zhao, Xing, Sun: "β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibition of renal fibroblast activation and fibrosis." in: Journal of pharmacological sciences, Vol. 133, Issue 4, pp. 203-213, 2017

Zhao, Fu, Yang, Dong, Yang, Sun, Hou: "Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro." in: Life sciences, Vol. 145, pp. 213-23, 2016

Motawi, Darwish, Hamed, El-Rigal, Naser: "A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats." in: Molecular neurobiology, 2016

Ahmad, Sattar, Rathore, Abdulla, Khan, Azam, Abdullah, Johns: "Up Regulation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol-Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150137, 2016

Zhang, Wang, Zhang, Zhu, Yu, Peng: "Pleiotropic effects of survivin in vascular endothelial cells." in: Microvascular research, Vol. 108, pp. 10-6, 2016

Giampá, Mônico-Neto, de Mello, Souza, Tufik, Lee, Koike, Dos Santos, Antonio, Serra, Tucci, Antunes: "Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training." in: PLoS ONE, Vol. 11, Issue 11, pp. e0167029, 2016

Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, 2016

Li, Zhang, Sun, Zhang, Han: "Angiotensin-(1-7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats." in: Journal of the American Society of Hypertension : JASH, Vol. 9, Issue 11, pp. 865-77, 2015

Li, Jiang, Chen, Liu, Geng, Guo, Sun, Zhu, Shan: "Renal sympathetic denervation improves cardiac dysfunction in rats with chronic pressure overload." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 64, Issue 5, pp. 653-62, 2015

Razga, Kovacs, Bódi, Talapka, Nyengaard: "Heterogeneous downregulation of angiotensin II AT1-A and AT1-B receptors in arterioles in STZ-induced diabetic rat kidneys." in: BioMed research international, Vol. 2014, pp. 947506, 2014

Wang, Shibamoto, Kuda, Sun, Tanida, Kurata: "Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats." in: European journal of pharmacology, Vol. 731, pp. 38-43, 2014

Gan, Sun, Chen, Zhang, Zhou, Chen, Zhou: "Intermedin in the paraventricular nucleus attenuates cardiac sympathetic afferent reflex in chronic heart failure rats." in: PLoS ONE, Vol. 9, Issue 4, pp. e94234, 2014

Sun, Zhou, Feng, Gao, Ding, Tang, Zhu, Zhou: "Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats." in: Acta physiologica (Oxford, England), Vol. 212, Issue 4, pp. 267-82, 2014

Sun, Li, Chen, Xiong, Han: "Angiotensin II and angiotensin-(1-7) in paraventricular nucleus modulate cardiac sympathetic afferent reflex in renovascular hypertensive rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e52557, 2013

Yuan, Zhang, Zhang, Gao, Zhou, Han, Zhu: "SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats." in: Pflügers Archiv : European journal of physiology, Vol. 465, Issue 2, pp. 261-70, 2013

Peng, Xing, Ye, Li, Luo, Li, Lou: "High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells." in: Molecular medicine reports, Vol. 9, Issue 2, pp. 450-6, 2013

Liu, Hu, Wang, Zhang, Ma, Feng, Wang, Wang, Dong, Gao, Zhang, Zhang: "Angiotensin-converting enzyme (ACE) 2 overexpression ameliorates glomerular injury in a rat model of diabetic nephropathy: a comparison with ACE inhibition." in: Molecular medicine (Cambridge, Mass.), Vol. 17, Issue 1-2, pp. 59-69, 2011

Nissim-Sabat, Farr, McCune, Stith: "Community mental health centers and insurance reimbursements." in: Community mental health journal, Vol. 22, Issue 2, pp. 160-5, 1986

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Produktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN416068) zurück nach oben
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ELISA image for Angiotensin II (Ang II) ELISA Kit (ABIN416068) Angiotensin II (Ang II) ELISA Kit