Human IgG isotype control (APC)

Details zu Produkt Nr. ABIN5633235, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Human
219
209
209
171
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Konjugat
APC
207
168
165
164
157
108
58
58
48
45
24
13
12
12
11
9
9
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Kreuzreaktivität (Details) Human IgG whole molecule Allophycocyanin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Allophycocyanin, anti-Human IgG and anti-Human Serum.
Reinigung Human IgG whole molecule Allophycocyanin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Labeling Ratio 4-6
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise

Flow Cytometry Dilution: 1:500-1:2500

Immunohistochemistry Dilution: 1:500-1:2500

Application Note: Human IgG whole molecule Allophycocyanin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Western Blot Dilution: 1:1,000-1:5,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Haltbarkeit 12 months