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Llama IgG isotype control (HRP)

ELISA, FACS, WB IgG HRP
Produktnummer ABIN5608820
  • Target Alle IgG Produkte
    IgG
    Reaktivität
    Llama
    Wirt
    • 144
    • 140
    • 126
    • 89
    • 89
    • 69
    • 57
    • 56
    • 47
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 14
    • 9
    • 9
    • 4
    • 2
    Llama
    Konjugat
    HRP
    Applikation
    ELISA, Flow Cytometry (FACS), Western Blotting (WB)
    Aufreinigung
    Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
    Isotyp
    IgG
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG2 HRP conjugated isotype control can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Kommentare

    Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
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