Rhesus Monkey IgA, IgG, IgM isotype control (HRP)

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Isotyp
Wirt
Rhesus Monkey
Klonalität
Polyklonal
Konjugat
HRP
Applikation
Immunocytochemistry (ICC), Isotype Control (IsoC), Immunohistochemistry (IHC), ELISA
Optionen
Hersteller
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Immunogen Purified immunoglobulin IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotyp IgG IgA IgM Mix
Fragment Fc fragment,Fab fragment
Spezifität The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes.
Produktmerkmale Horseradish peroxidase-conjugated IgG fraction of polyclonal monkey antiserum to rabbit immunoglobulins IgG, IgA and IgM, heavy and light chains
Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
Reinigung No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Andere Bezeichnung IgG + IgA + IgM
Applikationshinweise In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:8,000.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Konzentration 10 mg/mL
Buffer Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Konservierungsmittel Without preservative
Handhabung Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.