Hamster IgG isotype control (Biotin)

Details zu Produkt Nr. ABIN457793, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Hamster
219
209
209
172
162
129
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Klonalität
Polyklonal
Konjugat
Biotin
206
168
165
164
157
108
58
58
48
45
25
13
12
12
11
9
9
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Isotype Control (IsoC), Immunohistochemistry (IHC), ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Purified normal IgG isolated from pooled Syrian hamster serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotyp IgG
Fragment H&L
Spezifität The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule.
Produktmerkmale Biotin-conjugated IgG fraction of polyclonal hamster antiserum to hamster IgG, heavy and light chains
Biotin/IgG protein molar ratio (B/P) is approximately 4.5.
Reinigung No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of hamster origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:6,000.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Konzentration 10 mg/mL
Buffer Biotin-coupled purified hyperimmune hamster IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Konservierungsmittel Without preservative
Handhabung Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day . If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.