HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN2870554, Anbieter: Anmelden zum Anzeigen
Isotyp
Reaktivität
Human
Wirt
HEK-293 Cells
215
209
202
170
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
2
Biologische Aktivität
Active
Konjugat
AVI tag,Biotin,His tag
207
168
165
164
155
108
58
58
48
41
24
13
12
12
11
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Isotype Control (IsoC)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Isotyp IgG
Fragment Fc fragment
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28.8 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 60-66 kDa under non-reducing (NR) condition.
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Molekulargewicht 28.8 kDa
Gen-ID 18, 17
UniProt P01857
Forschungsgebiet Secondary Antibodies
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) (ABIN2870554) Biotinylated polyhistidine tagged human IgG1 Fc protein on SDS-PAGE under reducing (R...
Binding Studies (Bind) image for HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) (ABIN2870554) Immobilized Recombinant Protein A (Cat# RPA-S3149) at 2 μg/mL (100 µl/well),can bind ...