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HEK-293 Cells IgG2a Isotyp-Kontrolle

IsoC IgG2a unconjugated
Produktnummer ABIN2181277
  • Target Alle IgG2a Produkte
    IgG2a
    Reaktivität
    Maus
    Wirt
    • 183
    • 75
    • 2
    • 1
    HEK-293 Cells
    Applikation
    Isotype Control (IsoC)
    Produktmerkmale
    This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa under reducing (R) condition, and 50-60 kDa under non-reducing (NR) condition (SDS-PAGE).
    Reinheit
    >90 % as determined by SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
    Isotyp
    IgG2a
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    Buffer
    50 mM Tris, 100 mM Glycine, pH 7.5
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Target
    IgG2a
    Abstract
    IgG2a Produkte
    Synonyme
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Substanzklasse
    Antibody
    Hintergrund
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
    Molekulargewicht
    26.4 kDa
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