HEK-293 Cells IgG1 Isotyp-Kontrolle

Details zu Produkt Nr. ABIN2181273, Anbieter: Anmelden zum Anzeigen
Isotyp
  • IgG1
  • Igh-4
  • VH7183
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Ighg1
Reaktivität
Maus
Wirt
HEK-293 Cells
256
85
22
4
3
3
2
2
1
1
1
Biologische Aktivität
Active
Applikation
Isotype Control (IsoC)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Isotyp IgG1
Produktmerkmale This protein carries no "tag". The protein has a calculated MW of 24.3 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Reinheit >95 % as determined by SDS-PAGE.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Molekulargewicht 24.3 kDa
Forschungsgebiet Secondary Antibodies
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Bilder des Herstellers
SDS-PAGE (SDS) image for HEK-293 Cells IgG1 isotype control (ABIN2181273) Mouse IgG1 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained...
Allgemeine Veröffentlichungen den Hertog, Visser, Ingham, Fey, Klatser, Anthony: "Simplified automated image analysis for detection and phenotyping of Mycobacterium tuberculosis on porous supports by monitoring growing microcolonies." in: PLoS ONE, Vol. 5, Issue 6, pp. e11008, 2010 (PubMed).