Human IgG Isotyp-Kontrolle

Details zu Produkt Nr. ABIN1881039, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Human
219
209
209
171
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Klonalität
Polyklonal
Applikation
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Reinigung Protein A and peptide affinity chromatography
Hintergrund

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Molekulargewicht 150 kDa
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise WB(Assay-dependent)
ELISA(Assay-dependent)
FCM(Assay-dependent)
IHC-P(Assay-dependent)
IHC-F(Assay-dependent)
IF(IHC-P)(Assay-dependent)
IF(IHC-F)(Assay-dependent)
IF(ICC)(Assay-dependent)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Produkt verwendet in: Pi, Long, Huang, Cheng, Zheng: "A sandwich immunoassay for detection of Aβ(1-42) based on quantum dots." in: Talanta, Vol. 146, pp. 10-5, 2015 (PubMed).

Zhou, Gan, Li, Hu, Li, Wang, Zheng: "A cost-effective sandwich electrochemiluminescence immunosensor for ultrasensitive detection of HIV-1 antibody using magnetic molecularly imprinted polymers as capture probes." in: Biosensors & bioelectronics, Vol. 54, pp. 199-206, 2014 (PubMed). Von den Autoren verwendete Methode: ELISA (ELISA)

Gao, Wang, Wang, Han, Fu: "Amorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin." in: Analytica chimica acta, Vol. 819, pp. 102-7, 2014 (PubMed).

Li, Qiang, Wang, Vuki, Xu: "Ultrasensitive and fast fluorescent bioassay based on fluorescence enhancement of silver nanoparticles." in: The Analyst, Vol. 138, Issue 24, pp. 7376-83, 2013 (PubMed).