Human IgG Isotyp-Kontrolle

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Isotyp
Wirt
Human
219
209
209
171
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Applikation
Dot Blot (DB), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Isotype Control (IsoC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Fragment Whole molecule
Produktmerkmale Purified Human IgG (Whole Molecule) Control is purified from normal human serum through Protein G chromatography. Purified Human IgG (Whole Molecule) Control can be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Western and Dot blots), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reactions.
Reinigung Protein G chromatography
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Each Investigator should determine their own optimal working dilution for specific applications.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 4 mg/mL.
Konzentration 4 mg/mL
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Produkt verwendet in: van Zeijl, van Koppen, Surovtseva, de Gooyer, Plate, Conti, Karstens, Timmers, Saeed, Wiersinga, Miltenburg, Fliers, Boelen: "Complete inhibition of rhTSH-, Graves' disease IgG-, and M22-induced cAMP production in differentiated orbital fibroblasts by a low-molecular-weight TSHR antagonist." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 5, pp. E781-5, 2012 (PubMed).

Calvert, Kalantarov, Chang, Trakht, Blair, Roehrig: "Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein." in: Virology, Vol. 410, Issue 1, pp. 30-7, 2011 (PubMed).