Maus Immunoglobulin G1 (IgG1) Isotyp-Kontrolle
| Antigen |
|
| Synonyme |
IgG1, Igh-4, VH7183 |
| Klonalität |
Monoklonal (PPV-06) |
|
Wirt
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|
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Reaktivität
|
Maus
|
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Applikation
|
Durchflusszytometrie (FACS)
|
| Produktnummer |
ABIN125733 |
| Menge |
0.1 mg (1 mg/ml) (Varianten) |
| Preis |
155,00 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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IgG1
|
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Format
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Azide Free
|
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Isotyp
|
IgG1
|
|
Klon
|
PPV-06
|
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Beschreibung
|
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration(more than 15 µ g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control foreach specific monoclonal antibody used in a particular immunoassay. The monoclonalantibody PPV-06, generated against a plant pathogen, does not cross-react with otherspecies, and hence all the background that could be observed when working with thisantibody would be a result of general nonspecific interactions between an mouse IgG1molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
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Spezifität
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This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on aplant pathogen. This antibody does not react with: Human, Porcine, Mouse, Rat, Other species Not tested (expected negative).
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Sensitivität
|
The specificity of staining by monoclonal antibodies to target antigens should be verified by
establishing the amount of non-specific antibody binding. Especially at higher concentration
(more than 15 mg/ml) the antibody staining usually has consignable background. To this end
a non-reactive immunoglobulin of the same isotype is included as a negative control for
each specific monoclonal antibody used in a particular immunoassay. The monoclonal
antibody PPV-06, generated against a plant pathogen, does not cross-react with other
species, and hence all the background that could be observed when working with this
antibody would be a result of general nonspecific interactions between an mouse IgG1
molecule and the respective sample under the particular conditions. This shall help the
customer to set up the experimental conditions so that the nonspecific binding of any
antibody is abolished.
|
|
Applikationshinweise
|
Flow Cytometry. Control Experiments The antibody is suitable for control experiments when performing cell surface staining aswell as intracellular staining.
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Konzentration
|
1 mg/ml
|
|
Reinheit
|
> 95% (by SDS-PAGE)
|
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Reinigung
|
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
|
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Lagerung
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Sekundärantikörper
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Immunoglobulin G1 (IgG1)", Typ "Isotyp-Kontrollen" finden
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Wirte
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Maus (69), Ratte (33), Human (1)
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Konjugate
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FITC (12), Biotin (10), APC (9), PE (8), SPRD (5), APC,Cy7 (4), PE,Cy7 (4), APC,Cy5.5 (2), Alexa Fluor 488 (2), Alexa Fluor 700 (2), Cy5 (2), PE,Cy5.5 (2), Pacific Blue (2), RPE (2), Alexa Fluor 647 (1), DY647 (1), Dyomics 647 (1), PE,Texas Red (TR) (1), PE-Dyomics 647 (1), RPE,Cy5 (1)
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Alternativen 
