anti-Schaf FSHR Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended FSHR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Follicle Stimulating Hormone Receptor (FSHR) Antikörper
  • fshr
  • FSHR
  • fshr-A
  • GTHR-I
  • zfGTHR-I
  • fsh-r
  • LOC100304505
  • FSHRO
  • LGR1
  • ODG1
  • FSH-R
  • eFSHR
  • FSHRE
  • follicle stimulating hormone receptor
  • follicle stimulating hormone receptor pseudogene
  • follicle stimulating hormone receptor L homeolog
  • follicle-stimulating hormone receptor
  • fshr
  • LOC783409
  • FSHR
  • fshr.L
  • fsh-r
  • Bm1_17785
  • LOC100304505
  • Fshr
Epitop
N-Term
19
15
12
10
7
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Fledermaus, Rind (Kuh), Ziege, Pferd, Human, Affe, Schwein, Kaninchen, Ratte (Rattus), Schaf
175
58
53
5
Wirt
Kaninchen
102
75
Klonalität
Polyklonal
Konjugat
Dieser FSHR Antikörper ist unkonjugiert
7
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
83
78
68
50
41
28
21
13
8
5
2
2
1
1
Hersteller
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Bilder

Produktnummer ABIN1048616
$ 451.00
Zzgl. Versandkosten $45.00

General

Antigen Follicle Stimulating Hormone Receptor (FSHR) Antikörper
Epitop N-Term
(19), (15), (12), (10), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Fledermaus, Rind (Kuh), Ziege, Pferd, Human, Affe, Schwein, Kaninchen, Ratte (Rattus), Schaf
(175), (58), (53), (5)
Wirt Kaninchen
(102), (75)
Klonalität
Polyklonal
Konjugat Dieser FSHR Antikörper ist unkonjugiert
(7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(83), (78), (68), (50), (41), (28), (21), (13), (8), (5), (2), (2), (1), (1)
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Produktdetails anti-FSHR Antikörper

Target Details FSHR Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human FSHR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Sheep, Goat, Bat, Bovine, Rabbit, Horse, Pig (100%) Mouse, Elephant, Dog, Cat, Guinea pig, Sparrow (94%) Monkey, Hamster, Opossum (89%) Turkey (83%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FSHR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Sheep, Goat, Bat, Bovine, Rabbit, Horse, Pig (100%), Mouse, Elephant, Dog, Cat, Guinea pig, Sparrow (94%), Monkey, Hamster, Opossum (89%), Turkey (83%).

Type of Immunogen: Synthetic peptide

Target Details FSHR

Produktdetails anti-FSHR Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung FSH Receptor / FSHR (FSHR Antibody Abstract)
Hintergrund Name/Gene ID: FSHR
Subfamily: Glycoprotein hormone
Family: GPCR

Synonyms: FSHR, Follitropin receptor, FSHRO, Ovarian dysgenesis 1, FSH receptor, FSH-R, LGR1, ODG1
Gen-ID 2492
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling

Anwendungsinformationen

Produktdetails anti-FSHR Antikörper Target Details FSHR Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
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Handhabung

Produktdetails anti-FSHR Antikörper Target Details FSHR Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-FSHR Antikörper Target Details FSHR Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Follicle Stimulating Hormone Receptor (FSHR) (N-Term) antibody (ABIN1048616) Anti-FSHR antibody ABIN1048616 IHC staining of human ovary. Immunohistochemistry of ...