Caspase-Family Inhibitor Z-VAD-FMK

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Applikation
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Verwendungszweck A pan-caspase inhibitor
Sequenz Z-Val-Ala-Asp(OMe)-FMK
Produktmerkmale PubChem CID: 5737
A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Reinheit Single spot by TLC
Chemical Name Z-VAD-FMK, Z-VAD fluoromethylketone, Caspase-Family Inhibitor, pan-caspase inhibitor
Formel C₂₂H₃₀N₃O₇F
Permeabilität Cell-permeable
Löslichkeit DMSO
Molekulargewicht 467.5 g/mol
CAS-Nummer 187389-52-2
Applikationshinweise We recommend using 1000X dilutions for inhibiting caspase activity in Jurkat cell culture (e.g., Add 1 µL to 1 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 10 mM
Handhabung Protect from light and moisture
Lagerung -20 °C
Haltbarkeit 6-12 months
Bilder des Herstellers
 image for Caspase-Family Inhibitor Z-VAD-FMK (ABIN412347) Caspase-Family Inhibitor Z-VAD-FMK
Produkt verwendet in: Jouve, Despoix, Espeli, Gauthier, Cypowyj, Fallague, Schiff, Dignat-George, Vély, Leroyer: "The involvement of CD146 and its novel ligand Galectin-1 in apoptotic regulation of endothelial cells." in: The Journal of biological chemistry, Vol. 288, Issue 4, pp. 2571-9, 2013 (PubMed).

Liu, Deng, Lancto, Abrahamsen, Rutherford, Enomoto: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed).

Dai, Liu, Tang, DeSano, Burstein, Davis, Pienta, Lawrence, Xu: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed).

Liu, Enomoto, Lancto, Abrahamsen, Rutherford: "Inhibition of apoptosis in Cryptosporidium parvum-infected intestinal epithelial cells is dependent on survivin." in: Infection and immunity, Vol. 76, Issue 8, pp. 3784-92, 2008 (PubMed).

Arai, Miyake, Voit, Nemoto, Wakeland, Grummt, Miyazaki: "Death-effector domain-containing protein DEDD is an inhibitor of mitotic Cdk1/cyclin B1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2289-94, 2007 (PubMed).

Niikura, Dixit, Scott, Perkins, Kitagawa: "BUB1 mediation of caspase-independent mitotic death determines cell fate." in: The Journal of cell biology, Vol. 178, Issue 2, pp. 283-96, 2007 (PubMed).

Deng, Yin, Lu, Cai, Yin: "The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 91, Issue 1, pp. 59-69, 2006 (PubMed).

Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Pae, Choi, Oh, Lee, Ryu, Rhew, Kim, Chung: "Roles of heme oxygenase-1 in the antiproliferative and antiapoptotic effects of nitric oxide on Jurkat T cells." in: Molecular pharmacology, Vol. 66, Issue 1, pp. 122-8, 2004 (PubMed).

Song, Mahidhara, Zhou, Hoffman, Seol, Flavell, Billiar, Otterbein, Choi: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Fukazawa, Walter, Owen-Schaub: "Adenoviral Bid overexpression induces caspase-dependent cleavage of truncated Bid and p53-independent apoptosis in human non-small cell lung cancers." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25428-34, 2003 (PubMed).

Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Erdreich-Epstein, Tran, Bowman, Wang, Cabot, Durden, Vlckova, Reynolds, Stins, Groshen, Millard: "Ceramide signaling in fenretinide-induced endothelial cell apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49531-7, 2002 (PubMed).