anti-Hamster TRPV4 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended TRPV4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) Antikörper
  • wu:fp52e02
  • CMT2C
  • HMSN2C
  • OTRPC4
  • SMAL
  • SPSMA
  • SSQTL1
  • TRP12
  • VRL2
  • VROAC
  • 0610033B08Rik
  • Trp12
  • VR-OAC
  • VRL-2
  • Otrpc4
  • Vroac
  • transient receptor potential cation channel, subfamily V, member 4
  • transient receptor potential cation channel subfamily V member 4
  • trpv4
  • TRPV4
  • Trpv4
Epitop
Internal Region
19
18
7
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
94
52
52
6
6
5
4
3
2
2
1
Wirt
Kaninchen
97
6
1
Klonalität
Polyklonal
Konjugat
Dieser TRPV4 Antikörper ist unkonjugiert
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
31
29
15
13
4
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1049441
$ 451.00
Zzgl. Versandkosten $45.00

General

Antigen Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) Antikörper
Epitop Internal Region
(19), (18), (7), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
(94), (52), (52), (6), (6), (5), (4), (3), (2), (2), (1)
Wirt Kaninchen
(97), (6), (1)
Klonalität
Polyklonal
Konjugat Dieser TRPV4 Antikörper ist unkonjugiert
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(77), (31), (29), (15), (13), (4), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TRPV4 Antikörper

Target Details TRPV4 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human TRPV4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%) Elephant, Rabbit (95%) Turkey, Chicken, Platypus (90%) Xenopus (85%) Stickleback, Zebrafish (80%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from internal region of human TRPV4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%), Elephant, Rabbit (95%), Turkey, Chicken, Platypus (90%), Xenopus (85%), Stickleback, Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Target Details TRPV4

Produktdetails anti-TRPV4 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung TRPV4 (TRPV4 Antibody Abstract)
Hintergrund Name/Gene ID: TRPV4
Subfamily: Calcium channel - TRP
Family: Ion Channel

Synonyms: TRPV4, CMT2C, HMSN2C, OTRPC4, SSQTL1, VR-OAC, VRL-2, Osm-9-like TRP channel 4, SPSMA, TRP12, VRL2, VROAC, SMAL
Gen-ID 59341
Forschungsgebiet Neurology
Pathways Hormone Transport, Cell-Cell Junction Organization

Anwendungsinformationen

Produktdetails anti-TRPV4 Antikörper Target Details TRPV4 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (5 - 10 μg/mL), WB
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TRPV4 Antikörper Target Details TRPV4 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-TRPV4 Antikörper Target Details TRPV4 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) (Internal Region) antibody (ABIN1049441) Anti-TRPV4 antibody ABIN1049441 IHC staining of human pancreas, islet. Immunohistoch...