Ratte (Rattus) Interleukin 6 ELISA Kit

Recommended Interleukin 6 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Interleukin 6 (IL6) ELISA Kits
  • Il-6
  • ILg6
  • Ifnb2
  • BSF2
  • HGF
  • HSF
  • IFNB2
  • IL-6
  • CHIL-6
  • il6
  • interleukin 6
  • interleukin 6 (interferon, beta 2)
  • interleukin-6
  • Il6
  • IL6
  • il-6
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
99
63
48
24
23
19
19
17
15
11
10
10
9
5
4
4
2
1
Methodentyp
Sandwich ELISA
Detektionsbereich
30-10000 pg/mL
Untere Nachweisgrenze
30 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN625205
$ 476.30
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN416249 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.8-500 pg/mL 7.8 pg/mL Anmelden zum Anzeigen 61
10.090268 ABIN921064 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen 94
10.090268 ABIN2472111 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
10.090268 ABIN1979356 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
10.090268 ABIN1115674 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen 4
10.090268 ABIN491785 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.69-500 pg/mL 0.69 pg/mL Anmelden zum Anzeigen
10.090268 ABIN1570747 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Anmelden zum Anzeigen
10.090268 ABIN924838 Sandwich ELISA Cell Lysate, Serum, Plasma 39-5000 pg/mL 39 pg/mL Anmelden zum Anzeigen
10.090268 ABIN578858 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
10.090268 ABIN1888510 Sandwich ELISA Plasma, Cell Culture Cells 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2708663 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2708664 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2114336 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Anmelden zum Anzeigen
1 ABIN365191 Sandwich ELISA Plasma, Serum, Tissue Homogenate 0.312-20 pg/mL 0.312 pg/mL Anmelden zum Anzeigen 12
1 ABIN2801454 Sandwich ELISA Serum, Plasma 327.7-80000 pg/mL 327.7 pg/mL Anmelden zum Anzeigen
1 ABIN1873515 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.56-100 pg/mL 1.56 pg/mL Anmelden zum Anzeigen
1 ABIN3032874 Competition ELISA Cell Culture Supernatant, Plasma, Serum 40.9-10000 pg/mL 40.9 pg/mL Anmelden zum Anzeigen
1 ABIN627179 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Anmelden zum Anzeigen
1 ABIN5656033 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.69 pg/mL - 500 pg/mL 0.69 pg/mL Anmelden zum Anzeigen
1 ABIN5656034 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 7.8 pg/mL - 500 pg/mL 7.8 pg/mL Anmelden zum Anzeigen

General

Antigen Interleukin 6 (IL6) ELISA Kits
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(99), (63), (48), (24), (23), (19), (19), (17), (15), (11), (10), (10), (9), (5), (4), (4), (2), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 30-10000 pg/mL
Untere Nachweisgrenze 30 pg/mL
Applikation ELISA
Pubmed 20 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails Interleukin 6 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für Interleukin 6 Kit (ABIN625205) Bilder
Verwendungszweck Rat IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben Plasma, Cell Culture Supernatant, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Kreuzreaktivität (Details) This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha.
Sensitivität < 30 pg/mL
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Antigendetails

Produktdetails Interleukin 6 ELISA Kit Anwendungsinformationen Handhabung Referenzen für Interleukin 6 Kit (ABIN625205) Bilder zurück nach oben
Antigen
Andere Bezeichnung IL-6 (IL6 ELISA Kit Abstract)
Hintergrund Interleukin-6 (IL-6)
Gen-ID 24498
UniProt P20607
Forschungsgebiet Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Pathways Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis

Anwendungsinformationen

Produktdetails Interleukin 6 ELISA Kit Antigendetails Handhabung Referenzen für Interleukin 6 Kit (ABIN625205) Bilder zurück nach oben
Applikationshinweise Recommended Dilution for serum and plasma samples2 fold
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Aufbereitung der Reagenzien 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent C (Item L) should be used for dilution of serum/plasma/culture supernatants/urine. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B (Item E) should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C. Add 500 µL Assay Diluent C (Item L) into Item C vial to prepare a 10,000 pg/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Pipette 300 µL Assay Diluent C into each tube. Use the 10,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent C serves as the zero standard (0 pg/mL). 200 µL Standard (Item C) + 500 µL 200myl 200 µL 200 µL 200 µL 200 µL 10,000 4,000 1,600 640 256 102.4 40.96 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B (Item E) into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B (Item E). For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 30 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 400-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Testdurchführung 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Ergebnisberechnung Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent C Rat IL-6 concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 10 100 1,000 10,000
Sensitivity: The minimum detectable dose of IL-6 is typically less than 30 pg/mL.
Recovery: Recovery was determined by spiking various levels of IL-6 into normal rat serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 115.6 105-127 Plasma 122.8 109-138 Cell culture media 130.2 118-138
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 102.4 98.75 99.89 Range ( %) 91-112 88-117 88-107 1:4 Average % of Expected 104.6 87.33 96.96 Range ( %) 93-114 76-97 85-105
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Testpräzision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für Interleukin 6 Kit (ABIN625205) Bilder zurück nach oben
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months

Referenzen für Interleukin 6 Kit (ABIN625205)

Produktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Xu, Yang, Sun, Chen, Jiang, Zheng, Ding, Liu, Sheng, Cui, Duan: "2,3',4,4',5-Pentachlorobiphenyl Induces Inflammatory Responses in the Thyroid Through JNK and Aryl Hydrocarbon Receptor-Mediated Pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 149, Issue 2, pp. 300-11, 2016

Zhang, Zhang, Tsui: "Mesenteric lymph duct drainage attenuates acute lung injury in rats with severe intraperitoneal infection." in: Inflammation, Vol. 38, Issue 3, pp. 1239-49, 2015

Yaidikar, Thakur: "Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines, up-regulation of Bcl-2, down-regulation of Bax, and caspase-3." in: Molecular and cellular biochemistry, Vol. 402, Issue 1-2, pp. 141-8, 2015

Aydin, Halici, Albayrak, Polat, Karakus, Yildirim, Bayir, Cadirci, Ayan, Aksakal: "Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions." in: Basic & clinical pharmacology & toxicology, Vol. 117, Issue 3, pp. 173-9, 2015

Chen, Min, Wang, Leung, Shi, Zhou, Yu, Wang, An, Sha, Chen: "Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury." in: Scientific reports, Vol. 5, pp. 8718, 2015

Tang, Chen, Guo, Yang, Tao, Li, Miao, Feng, Chen, Zhu: "Minocycline Attenuates Neonatal Germinal-Matrix-Hemorrhage-Induced Neuroinflammation and Brain Edema by Activating Cannabinoid Receptor 2." in: Molecular neurobiology, 2015

Wang, Zhao, Han, Tang: "Protective effects of Semen Crotonis Pulveratum on trinitrobenzene sulphonic acid-induced colitis in rats and H?O?-induced intestinal cell apoptosis in vitro." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1699-707, 2015

Abdel Kawy: "Low-dose carvedilol protects against acute septic renal injury in rats during the early and late phases." in: Canadian journal of physiology and pharmacology, Vol. 93, Issue 6, pp. 443-50, 2015

Gao, Guan, Li, Su, Wang: "Ameliorating effects of low tidal volume ventilation with associated hypercapnia on pneumoperitoneum-induced lung injury by inhibition of Toll-like receptor 4." in: International journal of clinical and experimental medicine, Vol. 8, Issue 2, pp. 1814-23, 2015

Eleawa, Alkhateeb, Ghosh, Al-Hashem, Shatoor, Alhejaily, Khalil: "Coenzyme Q10 protects against acute consequences of experimental myocardial infarction in rats." in: International journal of physiology, pathophysiology and pharmacology, Vol. 7, Issue 1, pp. 1-13, 2015

Mohanty, Swamy, Middha, Prakash, Subbanarashiman, Maniyam et al.: "Analgesic, Anti- inflammatory, Anti- lipoxygenase Activity and Characterization of Three Bioactive Compounds in the Most Active Fraction of Leptadenia reticulata (Retz.)Wight & Arn. - A Valuable ..." in: Iranian journal of pharmaceutical research : IJPR, Vol. 14, Issue 3, pp. 933-42, 2015

Møller, Knudsen, Andersen, Nyengaard, Hamilton-Dutoit, Okholm Møller, Svendsen, Møller, Moestrup, Graversen, Mortensen: "Anti-CD163-dexamethasone protects against apoptosis after ischemia/reperfusion injuries in the rat liver." in: Annals of medicine and surgery (2012), Vol. 4, Issue 4, pp. 331-7, 2015

Shoeib, Said, Ammar: "Cytoprotective potential of tiron and methyl palmitate against acetaminophen-induced acute liver injury." in: Canadian journal of physiology and pharmacology, pp. 1-8, 2015

Salama, Al-Harbi, Abdel-Bakky, Omar: "Glutamyl cysteine dipeptide suppresses ferritin expression and alleviates liver injury in iron-overload rat model." in: Biochimie, Vol. 115, pp. 203-11, 2015

Iii Colado-Velázquez, Mailloux-Salinas, Medina-Contreras, Cruz-Robles, Bravo: "Effect of Serenoa Repens on Oxidative Stress, Inflammatory and Growth Factors in Obese Wistar Rats with Benign Prostatic Hyperplasia." in: Phytotherapy research : PTR, Vol. 29, Issue 10, pp. 1525-31, 2015

El-Gowilly, Helmy, El-Gowelli: "Pioglitazone ameliorates methotrexate-induced renal endothelial dysfunction via amending detrimental changes in some antioxidant parameters, systemic cytokines and Fas production." in: Vascular pharmacology, Vol. 74, pp. 139-50, 2015

Zou, Wen, Zheng, Xiao, Luo, Chen, Wang, Cheng, Xiang, Nie: "Metabolomic Study on the Preventive Effect of Patrinia scabiosaefolia Fisch on Multipathogen Induced Pelvic Inflammatory Disease in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 170792, 2015

Ikutomi, Sahara, Nakajima, Minami, Morita, Hirata, Komuro, Nakamura, Sata: "Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation." in: Journal of molecular and cellular cardiology, Vol. 86, pp. 121-35, 2015

Zou, Song, Duan, Hu, Cui, Gu: "Prolonged ischemia elicits acute allograft rejection involved in CXCR3 activation in rat kidney transplants." in: Transplant immunology, Vol. 33, Issue 2, pp. 103-9, 2015

He, Li, Zuo: "Nerve Blockage Attenuates Postoperative Inflammation in Hippocampus of Young Rat Model with Surgical Trauma." in: Mediators of inflammation, Vol. 2015, pp. 460125, 2015

Bilder

Produktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für Interleukin 6 Kit (ABIN625205) zurück nach oben
Bilder des Herstellers
ELISA image for Interleukin 6 (IL6) ELISA Kit (ABIN625205) Interleukin 6 (IL6) ELISA Kit