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|Applikation / Reaktivität||Huhn||Rind (Kuh)||Hund||Fisch||Ziege||Meerschweinchen||Hamster||Pferd||Human||Makake||Affe||Maus||Schwein||Ratte (Rattus)||Rhesusaffen||Schaf|
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|Antigen||Interleukin 6 (IL6) ELISA Kits|
Kits mit alternativen Reaktivitäten:
|Untere Nachweisgrenze||3.12 pg/mL|
|16 Publikationen vorhanden|
|Hersteller||Anmelden zum Anzeigen|
Produktdetails Interleukin 6 ELISA KitAntigendetails Anwendungsinformationen Handhabung ProductDetails: References for Interleukin 6 Kit (ABIN415997) Bilder
|Verwendungszweck||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL6 in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Proben||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
|Kreuzreaktivität (Details)||No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.|
AntigendetailsProduktdetails Interleukin 6 ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for Interleukin 6 Kit (ABIN415997) Bilder zurück nach oben
|Andere Bezeichnung||IL6 (IL6 ELISA Kit Abstract)|
|Forschungsgebiet||Cancer, Cytokines, Angiogenesis, Innate Immunity|
|Pathways||Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis|
AnwendungsinformationenProduktdetails Interleukin 6 ELISA Kit Antigendetails Handhabung ProductDetails: References for Interleukin 6 Kit (ABIN415997) Bilder zurück nach oben
Information on standard material:
|Protokoll||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
|Aufbereitung der Reagenzien||
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Aufbereitung der Proben||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with IL6 concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for Interleukin 6 Kit (ABIN415997) Bilder zurück nach oben
|Vorsichtsmaßnahmen||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
|Informationen zur Lagerung||
ProductDetails: References for Interleukin 6 Kit (ABIN415997)Produktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
|Produkt verwendet in:||
Mokra, Kosutova, Balentova, Adamkov, Mikolka, Mokry, Antosova, Calkovska: "Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavage model of acute lung injury." in: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, Vol. 67, Issue 6, pp. 919-932, 2017
Sozański, Kucharska, Rapak, Szumny, Trocha, Merwid-Ląd, Dzimira, Piasecki, Piórecki, Magdalan, Szeląg: "Iridoid-loganic acid versus anthocyanins from the Cornus mas fruits (cornelian cherry): Common and different effects on diet-induced atherosclerosis, PPARs expression and inflammation." in: Atherosclerosis, Vol. 254, pp. 151-160, 2016
Liu, Peng, Fang, Yao, Redmond, Ni: "No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy." in: Journal of orthopaedic surgery and research, Vol. 11, Issue 1, pp. 125, 2016
Mikolka, Kopincová, Košútová, Čierny, Čalkovská, Mokrá: "Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome." in: Physiological research, Vol. 65, Issue Supplementum 5, pp. S653-S662, 2016
Yu, Xie, Xin, Wang: "Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK." in: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 441-9, 2015
Castellani, Laufer, Melka, Diehl, OReilly, Singh: "DNA methylation differences in monozygotic twin pairs discordant for schizophrenia identifies psychosis related genes and networks." in: BMC medical genomics, Vol. 8, pp. 17, 2015
Lin, Chen, Lei, You, Huang, Luo, Li, Zhao, Yan: "Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis." in: Mediators of inflammation, Vol. 2015, pp. 364391, 2015
Marques, Teixeira, Aguas, Ribeiro, Costa-e-Silva, Ferreira: "Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD)." in: Veterinary research, Vol. 45, pp. 14, 2014
Chen, Lin, You, Lei, Li, Lin, Luo, Yan: "Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: implications in acute and chronic infections." in: Archives of oral biology, Vol. 59, Issue 10, pp. 1075-84, 2014
Fang, Ning, Waqar, Niimi, Li, Satoh, Shiomi, Ye, Dong, Fan: "Bisphenol A exposure enhances atherosclerosis in WHHL rabbits." in: PLoS ONE, Vol. 9, Issue 10, pp. e110977, 2014
Hoekstra, van Lienden, Verheij, van der Loos, Heger, van Gulik: "Enhanced tumor growth after portal vein embolization in a rabbit tumor model." in: The Journal of surgical research, Vol. 180, Issue 1, pp. 89-96, 2013
Sakuramoto, Shimojo, Jesmin, Unoki, Kamiyama, Oki, Miya, Kawano, Mizutani: "Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS." in: BMC anesthesiology, Vol. 13, Issue 1, pp. 47, 2013
Moon, Joe, Kwon, Choi, Park, Kim: "Notochordal cells influence gene expression of inflammatory mediators of annulus fibrosus cells in proinflammatory cytokines stimulation." in: Journal of Korean Neurosurgical Society, Vol. 48, Issue 1, pp. 1-7, 2010
Ke, Ding, Kong, Gao, Wang, Cheng, Sun: "Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma." in: Journal of translational medicine, Vol. 8, pp. 73, 2010
Rolland, Baliga, Rice, Kerstein: "Lower limb embolus: a near-lethal disease after age 75 years." in: Southern medical journal, Vol. 82, Issue 2, pp. 178-81, 1989
Miroshnikov, Reznikov, Kokhanov: "[Comparative evaluation of the action of serum proteins on tissue regeneration]." in: Biulleten' eksperimental'no? biologii i meditsiny, Vol. 103, Issue 1, pp. 95-7, 1987
BilderProduktdetails Interleukin 6 ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for Interleukin 6 Kit (ABIN415997) zurück nach oben
|Bilder des Herstellers||