anti-Human KI-67 Antikörper für Immunohistochemistry

Recommended KI-67 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Epitop
AA 1800-1850, Internal Region
91
54
50
18
18
15
15
14
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
457
112
72
17
16
9
4
2
1
Wirt
Kaninchen
260
158
32
9
5
3
Klonalität
Polyklonal
Konjugat
Dieser KI-67 Antikörper ist unkonjugiert
17
13
11
10
10
8
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
183
157
120
118
104
84
72
16
13
13
11
6
3
3
3
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN409934
361,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
8.900272 ABIN409932 ICC FACS IHC (fro) IF IHC (p) IHC WB Rabbit AA 1600-1700, Internal Region Anmelden zum Anzeigen Polyclonal 31
8.900272 ABIN409940 ICC IF IHC IHC (fro) IHC (p) Rabbit AA 1200-1300, Internal Region Anmelden zum Anzeigen Polyclonal 8
8.900272 ABIN409942 IHC (p) IHC Rabbit AA 2100-2200, Internal Region Anmelden zum Anzeigen Polyclonal 1
8.900272 ABIN152984 ICC IHC (fro) IF IHC (p) IHC WB Rabbit IgG Internal Region, AA 1550-1700 Anmelden zum Anzeigen Polyclonal 58
8.900272 ABIN269401 ICC IF IHC IHC (fro) IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 7
8.900272 ABIN2452458 IHC WB Mouse IgG2b AA 1-250 Anmelden zum Anzeigen 3D11 1
8.900272 ABIN969232 IHC ELISA Mouse IgG2b Anmelden zum Anzeigen 4A1 2
8.900272 ABIN969234 IHC ELISA WB Mouse IgG1 Anmelden zum Anzeigen 8D5 2
8.900272 ABIN5012938 FACS ICC IHC Rabbit IgG Anmelden zum Anzeigen 1297A
8.900272 ABIN1533330 IF IHC ELISA Rabbit IgG Anmelden zum Anzeigen Polyclonal
8.900272 ABIN3181134 IHC Mouse IgG1 Anmelden zum Anzeigen 4A8
8.900272 ABIN1873700 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
8.900272 ABIN3022897 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
8.900272 ABIN398995 IHC Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
8.900272 ABIN932511 IHC ELISA Mouse IgG2b Anmelden zum Anzeigen 4A1
8.900272 ABIN1844200 IHC ELISA Mouse IgG2b Anmelden zum Anzeigen
8.900272 ABIN1844201 IHC ELISA WB Mouse IgG1 Anmelden zum Anzeigen
8.900272 ABIN5550445 IHC Mouse IgG Anmelden zum Anzeigen IHC067
8.900272 ABIN5550434 IHC Mouse IgG Anmelden zum Anzeigen IHC067
8.900272 ABIN1848646 ELISA IF/ICC IHC Rabbit C-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) Antikörper
Epitop AA 1800-1850, Internal Region
(91), (54), (50), (18), (18), (15), (15), (14), (8), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(457), (112), (72), (17), (16), (9), (4), (2), (1)
Wirt Kaninchen
(260), (158), (32), (9), (5), (3)
Klonalität
Polyklonal
Konjugat Dieser KI-67 Antikörper ist unkonjugiert
(17), (13), (11), (10), (10), (8), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(187), (183), (157), (120), (118), (104), (84), (72), (16), (13), (13), (11), (6), (3), (3), (3), (1), (1), (1), (1)
Pubmed 12 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-KI-67 Antikörper

Target Details KI-67 Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN409934) Bilder
Reinigung Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the mouse Ki67 protein, within residues 1800-1850. [UniProt# P46013]

Target Details KI-67

Produktdetails anti-KI-67 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN409934) Bilder zurück nach oben
Antigen
Andere Bezeichnung Ki-67/MKI67 (MKI67 Antibody Abstract)
Hintergrund Gene Symbol: MKI67
Molekulargewicht Theoretical MW: 359 kDa
Gen-ID 4288
UniProt P46013
Pathways Glycosaminoglycan Metabolic Process

Anwendungsinformationen

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN409934) Bilder zurück nach oben
Applikationshinweise Western Blot, Flow Cytometry, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-FrozenThis Ki67 antibody is useful for Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. Immunohistochemistry-Frozen and Western Blot were reported in scientific literature. Use in FLOW cytometry reported in scientific literature ( PMID 25733567) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry Protocol specific for Ki67 Antibody Antigen UnmaskingBring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes.Wash sections in dH2O three times for 5 minutes each.Wash section in wash buffer for 5 minutes.Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature.Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C.Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature.Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.Wash sections three times in wash buffer for 5 minutes each.Add 100-400 µL DAB substrate to each section and monitor staining closely.As soon as the sections develop, immerse slides in dH2O.Wash sections in dH2O two times for 5 minutes each.Mount coverslips.Immunocytochemistry/Immunofluorescence Protocol for Ki67 antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen ProductDetails: References for anti-KI-67 antibody (ABIN409934) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer TBS and 0.1 % BSA
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.

ProductDetails: References for anti-KI-67 antibody (ABIN409934)

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Rodriguez, Siwko, Zeng, Li, Yi, Liu: "Prostate-specific G-protein-coupled receptor collaborates with loss of PTEN to promote prostate cancer progression." in: Oncogene, Vol. 35, Issue 9, pp. 1153-62, 2016

Yoon, Leyva-Castillo, Wang, Galand, Oyoshi, Kumar, Hoff, He, Chervonsky, Oppenheim, Kuchroo, van den Brink, Malefyt, Tessier, Fuhlbrigge, Rosenstiel, Terhorst, Murphy, Geha: "IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2147-66, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Gkouskou, Ioannou, Pavlopoulos, Georgila, Siganou, Nikolaidis, Kanellis, Moore, Papadakis, Kardassis, Iliopoulos, McDyer, Drakos, Eliopoulos: "Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis." in: Oncogene, Vol. 35, Issue 19, pp. 2496-505, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Kato, Hayashi, Kitahara, Nagasawa, Aono, Shibata, Utsumi, Amagase, Kadowaki: "Saireito (TJ-114), a Japanese traditional herbal medicine, reduces 5-fluorouracil-induced intestinal mucositis in mice by inhibiting cytokine-mediated apoptosis in intestinal crypt cells." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116213, 2015 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Talaber, Tuckermann, Okret: "ACTH controls thymocyte homeostasis independent of glucocorticoids." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 6, pp. 2526-34, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Flow Cytometry

He, Zhao, Yang, OConnell, Zhang, Oh, Ma, Lee, Zhang, Varghese, Yip, Dolatshahi Pirooz, Li, Zhang, Li, Ellen Martin, Machida, Liang: "Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers." in: Nature communications, Vol. 6, pp. 7839, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Liou, Teng, Tsai, Lin, Chen: "UV-blocking spectacle lens protects against UV-induced decline of visual performance." in: Molecular vision, Vol. 21, pp. 846-56, 2015

Jo, Yun, Park, Hee Park, Myoung Kim, Ho Pak, Jae Lee, Moon, Park, Song, Lee, Bae Han, Tae Hong: "Lung tumor growth-promoting function of peroxiredoxin 6." in: Free radical biology & medicine, Vol. 61, pp. 453-63, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Ifkovits, Addis, Epstein, Gearhart: "Inhibition of TGFβ signaling increases direct conversion of fibroblasts to induced cardiomyocytes." in: PLoS ONE, Vol. 9, Issue 2, pp. e89678, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Rodriguez, Luo, Weng, Zeng, Yi, Siwko, Liu: "PSGR promotes prostatic intraepithelial neoplasia and prostate cancer xenograft growth through NF-κB." in: Oncogenesis, Vol. 3, pp. e114, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Mobley, Bryant, Richard, Brann, Firestein, Greer: "Age-dependent regional changes in the rostral migratory stream." in: Neurobiology of aging, Vol. 34, Issue 7, pp. 1873-81, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Lin, Chang, Yang, Huang, Liu, Chang, Lin, Chen: "Assessment of ultraviolet B-blocking effects of weekly disposable contact lenses on corneal surface in a mouse model." in: Molecular vision, Vol. 19, pp. 1158-68, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Bilder

Produktdetails anti-KI-67 Antikörper Target Details KI-67 Anwendungsinformationen Handhabung ProductDetails: References for anti-KI-67 antibody (ABIN409934) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1800-1850), (Internal Region) antibody (ABIN409934) Immunohistochemistry-Paraffin: Ki67 Antibody [ABIN4099349] - FFPE section of human br...
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1800-1850), (Internal Region) antibody (ABIN409934) Immunohistochemistry: Ki67 Antibody [ABIN4099349] - Immunohistochemical analysis of m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1800-1850), (Internal Region) antibody (ABIN409934) Immunohistochemistry-Paraffin: Ki67 Antibody [ABIN4099349] - IHC analysis of Ki67 in ...
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1800-1850), (Internal Region) antibody (ABIN409934) Immunohistochemistry: Ki67 Antibody [ABIN4099349] - Detection of Ki67 in formalin-fix...
Immunofluorescence (IF) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1800-1850), (Internal Region) antibody (ABIN409934) Immunocytochemistry/Immunofluorescence: Ki67 Antibody [ABIN4099349] - Ki67 antibody w...