β-Glucosyltransferase enzyme

Details zu Produkt Nr. ABIN2669404, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Bacteriophage T4
Wirt
Escherichia coli (E. coli)
Applikation
Activity Assay (AcA), Tet-assisted Bisulfite Sequencing (TAB-Seq)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale β-Glucosyltransferase will transfer the glucose moiety from the UDP-Glucose (uridine diphosphoglucose) to 5-hydroxymethylcytosine residues in double-stranded DNA.
Bestandteile 200,000 Units β-Glucosyltransferase enzyme
10X β-Glucosyltransferase Reaction Buffer
50 mM UDP-Glucose
1 M DTT
Applikationshinweise The β-Glucosyltransferase enzyme can be used to glucosylate 5-hmC DNA. Glucosylated DNA can be used as a differentiator with glucosyl-sensitive restriction enzymes. The use of a labeled [14C] UDP-Glucose donor would enable labeling of the 5-hmC residues.
Kommentare

One unit is defined as the amount of enzyme required to fully protect 0.5 µg of 5-hmC DNA Standard (Catalog No. 55008) in 1 hour at 37°C in a total reaction volume of 50 µl from cleavage by 4 units of GlaI restriction enzyme in an incubation of 16 hours at 30°C. A pilot titration of enzyme is recommended for protection of genomic DNA.

Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C/-80 °C
Informationen zur Lagerung Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Bilder des Herstellers
Restriction Digest (RD) image for β-Glucosyltransferase enzyme (ABIN2669404) Figure 1: 0.5 µg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated...