anti-Meerschweinchen Lysyl Oxidase-Like 2 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Lysyl Oxidase-Like 2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Lysyl Oxidase-Like 2 (LOXL2) Antikörper
  • CG4402
  • Dmel\\CG4402
  • Dmloxl-2
  • dmlox-2
  • GB13360
  • lox2-like
  • LOXL2
  • lor2
  • loxl-2
  • ws9-14
  • LOR2
  • WS9-14
  • 1110004B06Rik
  • 4930526G11Rik
  • 9430067E15Rik
  • lysyl oxidase-like 2
  • lysyl oxidase homolog 4
  • lysyl oxidase like 2 L homeolog
  • lysyl oxidase like 2
  • lox2
  • LOC408544
  • LOXL2
  • loxl2.L
  • loxl2
  • Loxl2
Epitop
Internal Region
19
19
7
6
6
5
5
5
5
5
4
3
3
3
2
2
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
77
32
19
5
4
4
4
3
3
2
1
1
1
1
1
Wirt
Kaninchen
94
14
Klonalität
Polyklonal
Konjugat
Unkonjugiert
11
9
4
2
2
2
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
79
47
27
18
10
5
5
5
1
1
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1049011
$ 451.00
Zzgl. Versandkosten $45.00

General

Antigen Lysyl Oxidase-Like 2 (LOXL2) Antikörper
Epitop Internal Region
(19), (19), (7), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
(77), (32), (19), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Wirt Kaninchen
(94), (14)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(11), (9), (4), (2), (2), (2)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(79), (47), (27), (18), (10), (5), (5), (5), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human LOXL2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Guinea pig (100%) Bat (95%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from internal region of human LOXL2. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Guinea pig (100%), Bat (95%).

Type of Immunogen: Synthetic peptide

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung LOXL2 (LOXL2 Antibody Abstract)
Hintergrund Name/Gene ID: LOXL2

Synonyms: LOXL2, Lysyl oxidase-like protein 2, Lysyl oxidase related 2, Lysyl oxidase-like 2, WS9-14, LOR2, Lysyl oxidase homolog 2
Gen-ID 4017
Forschungsgebiet Cancer, Extracellular Matrix
Pathways Chromatin Binding

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (15 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Lysyl Oxidase-Like 2 (LOXL2) (Internal Region) antibody (ABIN1049011) Anti-LOXL2 antibody ABIN1049011 IHC staining of human liver. Immunohistochemistry of...
Immunohistochemistry (IHC) image for anti-Lysyl Oxidase-Like 2 (LOXL2) (Internal Region) antibody (ABIN1049011) Anti-LOXL2 antibody ABIN1049011 IHC staining of human prostate. Immunohistochemistry...