Maus Lipocalin 2 ELISA Kit

Recommended Lipocalin 2 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Lipocalin 2 (LCN2) ELISA Kits
  • LCN2
  • 24p3
  • MSFI
  • NGAL
  • AW212229
  • Sip24
  • lipocalin 2
  • lipocalin 2-like
  • LCN2
  • Lcn2
  • LOC100352980
Epitop
AA 21-200
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
48
32
32
14
8
8
4
3
3
2
2
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
78-5000 pg/mL
Untere Nachweisgrenze
78 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN411402
$ 400.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN1571485 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.625-40 ng/mL 0.625 ng/mL Anmelden zum Anzeigen
10.090268 ABIN366419 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Urine 12.5-800 pg/mL 12.5 pg/mL Anmelden zum Anzeigen
10.090268 ABIN415461 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Urine, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen
10.090268 ABIN492280 Sandwich ELISA Serum, Plasma, Urine, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 27.4-20.000 pg/mL 27.4 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2703298 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.02-100 ng/mL 0.02 ng/mL Anmelden zum Anzeigen
10.090268 ABIN2708581 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2114479 Cell Culture Supernatant, Serum, Plasma (heparin), Urine Anmelden zum Anzeigen
10.090268 ABIN2487178 Sandwich ELISA Plasma, Cell Culture Cells Anmelden zum Anzeigen
1 ABIN3032825 Competition ELISA Cell Culture Supernatant, Plasma, Serum 0.024-100 ng/mL 0.024 ng/mL Anmelden zum Anzeigen
1 ABIN5657367 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine 78.12 pg/mL - 20000 pg/mL 78.12 pg/mL Anmelden zum Anzeigen
1 ABIN5657368 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine 0.156 ng/mL - 10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN773463 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 100-2500 pg/mL 100 pg/mL Anmelden zum Anzeigen
1 ABIN1116191 Sandwich ELISA Serum, Plasma, Biological Fluids 39.063-2500 pg/mL 39.063 pg/mL Anmelden zum Anzeigen
1 ABIN2684845 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum, Urine 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen 2
1 ABIN5656452 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.024 ng/mL - 100 ng/mL 0.024 ng/mL Anmelden zum Anzeigen
1 ABIN2114639 Sandwich ELISA Serum, Plasma, Urine Anmelden zum Anzeigen
1 ABIN773360 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Anmelden zum Anzeigen
1 ABIN5521054 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 39.063-2500 pg/mL 39.063 pg/mL Anmelden zum Anzeigen
1 ABIN2192788 Sandwich ELISA Urine, Plasma, Serum, Tissue Culture Supernatant 10-1000 pg/mL 10 pg/mL Anmelden zum Anzeigen
1 ABIN4885773 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Anmelden zum Anzeigen

General

Antigen Lipocalin 2 (LCN2) ELISA Kits
Epitop AA 21-200
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(48), (32), (32), (14), (8), (8), (4), (3), (3), (2), (2), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 78-5000 pg/mL
Untere Nachweisgrenze 78 pg/mL
Applikation ELISA
Pubmed 6 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Lipocalin-2/NGAL
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Urine
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: NSO
Immunogen sequence: Q21-N200
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q21-N200

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung LCN2 (LCN2 ELISA Kit Abstract)
Hintergrund

Protein Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .

Background: Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Lipocalin-2,SV-40-induced 24P3 protein,Siderocalin LCN2,p25,Lcn2,

Full Gene Name: Neutrophil gelatinase-associated lipocalin

Cellular Localisation: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
Gen-ID 16819
UniProt P11672
Pathways Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Detected in lung, spleen, uterus, vagina and epididymis. .

Plattentyp Pre-coated
Protokoll mouse NGAL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGAL has been precoated onto 96-well plates. Standards(NSO, Q21-N200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGAL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGAL amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse NGAL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse NGAL standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 35.84, CV(%): 6.8
  • Sample 2: n=16, Mean(pg/ml): 1729, Standard deviation: 77.81, CV(%): 4.5
  • Sample 3: n=16, Mean(pg/ml): 3028, Standard deviation: 148.4, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 722, Standard deviation: 53.43, CV(%): 7.4
  • Sample 2: n=24, Mean(pg/ml): 2011, Standard deviation: 104.6, CV(%): 5.2
  • Sample 3: n=24, Mean(pg/ml): 3316, Standard deviation: 199, CV(%): 6
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Liu, Dong, Sun, Liu: "A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 919-26, 2014

Sun, Meng, Jiang, Liu, Lei, Su, Duan, Wu, Xia, Xia: "Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e80859, 2013

Koca, Olguner, Ergür, Altekin, Ta?dö?en, Duru, Girgin, Gündüz, Cilaker M?c?l?, Güzelda?, Akku?: "The effects of dexmedetomidine on secondary acute lung and kidney injuries in the rat model of intra-abdominal sepsis." in: TheScientificWorldJournal, Vol. 2013, pp. 292687, 2013

Olguner, Koca, Altekin, Ergür, Duru, Girgin, Ta?dö?en, Gündüz, Güzelda?, Akku?, Micili: "Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis." in: Experimental and therapeutic medicine, Vol. 5, Issue 6, pp. 1581-1588, 2013

Allgemeine Veröffentlichungen

Goetz, Holmes, Borregaard, Bluhm, Raymond, Strong: "The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition." in: Molecular cell, Vol. 10, Issue 5, pp. 1033-43, 2002

Cowland, Borregaard: "Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans." in: Genomics, Vol. 45, Issue 1, pp. 17-23, 1997

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
ELISA image for Lipocalin 2 (LCN2) ELISA Kit (ABIN411402) Mouse Lipocalin-2/NGAL PicoKine ELISA Kit standard curve