anti-Ratte (Rattus) Occludin Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Occludin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Occludin (OCLN) Antikörper
  • tpmt
  • OCLN
  • BLCPMG
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • occludin
  • occludin a
  • OCLN
  • ocln
  • Ocln
  • ocln-b
  • oclna
Epitop
AA 480-520
34
14
13
7
7
6
5
5
4
4
3
3
2
2
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
115
56
50
17
16
4
4
4
3
2
2
2
Wirt
Kaninchen
86
33
3
Klonalität
Polyklonal
Konjugat
Dieser Occludin Antikörper ist unkonjugiert
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
104
55
29
25
24
12
10
7
3
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN687337
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.588177 ABIN337048 IF IHC (p) ELISA WB Mouse kappa, IgG1 C-Term Anmelden zum Anzeigen OC-3F10 1
13.588177 ABIN4341292 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 1
13.588177 ABIN305888 IF IHC (p) IP ELISA WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN768875 IHC (p) WB Rabbit AA 35-84 Anmelden zum Anzeigen Polyclonal
1 ABIN5555079 EIA IF IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN687346 IHC (p) WB HRP Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687339 IHC (p) WB Biotin Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN4341291 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Occludin (OCLN) Antikörper
Epitop AA 480-520
(34), (14), (13), (7), (7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (1), (1)
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(115), (56), (50), (17), (16), (4), (4), (4), (3), (2), (2), (2)
Wirt Kaninchen
(86), (33), (3)
Klonalität
Polyklonal
Konjugat Dieser Occludin Antikörper ist unkonjugiert
(6), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(104), (55), (29), (25), (24), (12), (10), (7), (3), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Occludin Antikörper

Target Details Occludin Anwendungsinformationen Handhabung Referenzen für anti-Occludin Antikörper (ABIN687337) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse Occludin
Isotyp IgG

Target Details Occludin

Produktdetails anti-Occludin Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Occludin Antikörper (ABIN687337) Bilder zurück nach oben
Antigen
Andere Bezeichnung Occludin (OCLN Antibody Abstract)
Hintergrund

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular location: Cytoplasm

Synonyms: Ocl, AI53564, Occludin, Ocln

Gen-ID 18260
UniProt Q61146
Pathways Cell-Cell Junction Organization, Hepatitis C

Anwendungsinformationen

Produktdetails anti-Occludin Antikörper Target Details Occludin Handhabung Referenzen für anti-Occludin Antikörper (ABIN687337) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen Referenzen für anti-Occludin Antikörper (ABIN687337) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-Occludin Antikörper (ABIN687337)

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, 2015

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, 2014

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, 2013

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, 2012

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, 2011

Bilder

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen Handhabung Referenzen für anti-Occludin Antikörper (ABIN687337) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (IHC) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
Immunofluorescence (IF) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
'Independent Validation' Siegel
Chargennummer 990698W
Validierte Anwendung Immunofluorescence
Positivkontrolle Mouse stomach tissue
Negativkontrolle Human fat tissue
Bewertung Signal was detected in positive control sample and not in negative control sample.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: PPFE mouse stomach tissue
  • Negative Control: PPFE human fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul , Bioss bs-0295p
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only.
Protokoll
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Anmerkungen
  • No challenges notes
  • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
Validierungsbilder
Immunofluorescence validation image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (...