anti-Maus TJP1 Antikörper für Flow Cytometry

Recommended TJP1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Epitop
AA 886-995
16
10
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
94
34
32
20
10
5
4
4
3
2
1
1
1
1
Wirt
Kaninchen
76
16
3
3
2
Klonalität
Polyklonal
Konjugat
Dieser TJP1 Antikörper ist unkonjugiert
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
76
25
23
23
23
14
13
10
10
9
7
3
2
1
1
Hersteller
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Produktnummer ABIN1109613
$ 500.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2887986 FACS IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN4915355 FACS PE Rabbit IgG AA 1670-1720 Anmelden zum Anzeigen Polyclonal

General

Antigen Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
Epitop AA 886-995
(16), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(94), (34), (32), (20), (10), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Wirt Kaninchen
(76), (16), (3), (3), (2)
Klonalität
Polyklonal
Konjugat Dieser TJP1 Antikörper ist unkonjugiert
(3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(76), (25), (23), (23), (23), (14), (13), (10), (10), (9), (7), (3), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TJP1 Antikörper

Target Details TJP1 Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN1109613) Bilder
Spezifität This antibody recognizes specifically ZO-1 alpha-plus.
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse, Rat, Dog.
Reinigung Protein A Chromatography
Immunogen Amino acids 886-995 of human ZO-1
Isotyp Ig

Target Details TJP1

Produktdetails anti-TJP1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN1109613) Bilder zurück nach oben
Antigen
Andere Bezeichnung ZO-1 / Tight Junction Protein 1 (TJP1 Antibody Abstract)
Hintergrund Zona occludens 1 (ZO-1) is an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1
Gen-ID 7082
NCBI Accession NP_003248
UniProt Q07157
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization

Anwendungsinformationen

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Handhabung Referenzen für anti-TJP1 Antikörper (ABIN1109613) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Referenzen für anti-TJP1 Antikörper (ABIN1109613) Bilder zurück nach oben
Konzentration 0.1 mg/mL
Buffer PBS, 0.02 % Sodium Azide, 0.1 % BSA
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 2-8 °C.

Referenzen für anti-TJP1 Antikörper (ABIN1109613)

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Pelletier, Okawara, Vitale, Anderson: "Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpha+ and alpha- in guinea pig Sertoli cells: a possible association with F-actin and G-actin." in: Biology of reproduction, Vol. 57, Issue 2, pp. 367-76, 1997

Van Itallie, Balda, Anderson: "Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells." in: Journal of cell science, Vol. 108 ( Pt 4), pp. 1735-42, 1995

Balda, Anderson: "Two classes of tight junctions are revealed by ZO-1 isoforms." in: The American journal of physiology, Vol. 264, Issue 4 Pt 1, pp. C918-24, 1993

Willott, Balda, Heintzelman, Jameson, Anderson: "Localization and differential expression of two isoforms of the tight junction protein ZO-1." in: The American journal of physiology, Vol. 262, Issue 5 Pt 1, pp. C1119-24, 1992

Kurihara, Anderson, Farquhar: "Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 15, pp. 7075-9, 1992