anti-Human TJP1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended TJP1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Epitop
AA 1670-1720
15
10
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
94
34
32
19
11
5
4
4
3
2
1
1
1
1
Wirt
Kaninchen
76
16
3
3
2
Klonalität
Polyklonal
Konjugat
Dieser TJP1 Antikörper ist unkonjugiert
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
77
25
24
23
23
13
12
11
10
9
8
3
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN675024
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.591716 ABIN305736 IHC (fro) IHC (p) IP ELISA WB Rabbit IgG AA 463-1109 Anmelden zum Anzeigen Polyclonal
1 ABIN3043312 IHC (p) WB Rabbit IgG AA 1178-1527 Anmelden zum Anzeigen Polyclonal
1 ABIN650980 FACS IHC (p) WB Rabbit Ig AA 1570-1600, C-Term Anmelden zum Anzeigen Polyclonal 2
1 ABIN1109613 IHC (fro) FACS IF IHC (p) WB Rabbit Ig AA 886-995 Anmelden zum Anzeigen Polyclonal 5
1 ABIN768764 IHC (p) WB Rabbit AA 935-984 Anmelden zum Anzeigen Polyclonal
1 ABIN1109612 IHC (fro) IF IHC (p) WB Guinea Pig Ig Anmelden zum Anzeigen Polyclonal 3
1 ABIN4359735 ICC IF IHC IHC (p) SimWes Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
1 ABIN675033 IHC (p) HRP Rabbit IgG AA 1670-1720 Anmelden zum Anzeigen Polyclonal
1 ABIN4359733 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 2
1 ABIN675026 IHC (p) Biotin Rabbit IgG AA 1670-1720 Anmelden zum Anzeigen Polyclonal
1 ABIN2964417 IF IHC (p) WB Goat IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2629248 IHC (p) WB Rabbit IgG AA 1178-1527 Anmelden zum Anzeigen Polyclonal
1 ABIN4359734 ICC IF IHC IHC (p) SimWes Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN3029131 IHC (p) WB Rabbit IgG AA 1178-1527 Anmelden zum Anzeigen Polyclonal
1 ABIN2629246 IF IHC (p) WB Goat IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2629247 ICC IHC (p) WB Rabbit IgG AA 1-296 Anmelden zum Anzeigen Polyclonal
1 ABIN5536429 FACS IHC (p) WB Rabbit Ig Fraction AA 1570-1600, C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2964416 ICC IHC (p) WB Rabbit IgG AA 1-296 Anmelden zum Anzeigen Polyclonal
1 ABIN4359732 ICC IF IHC IHC (fro) IHC (p) WB Guinea Pig Anmelden zum Anzeigen Polyclonal
1 ABIN4359731 ICC IF IHC IHC (fro) IHC (p) WB Guinea Pig Anmelden zum Anzeigen Polyclonal

General

Antigen Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antikörper
Epitop AA 1670-1720
(15), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(94), (34), (32), (19), (11), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Wirt Kaninchen
(76), (16), (3), (3), (2)
Klonalität
Polyklonal
Konjugat Dieser TJP1 Antikörper ist unkonjugiert
(3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(77), (25), (24), (23), (23), (13), (12), (11), (10), (9), (8), (3), (2), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TJP1 Antikörper

Target Details TJP1 Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN675024) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human ZO-1
Isotyp IgG

Target Details TJP1

Produktdetails anti-TJP1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN675024) Bilder zurück nach oben
Antigen
Andere Bezeichnung Zo-1 (TJP1 Antibody Abstract)
Hintergrund

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Subcellular location: Cytoplasm

Synonyms: ZO-1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1

Gen-ID 7082
UniProt Q07157
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization

Anwendungsinformationen

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Handhabung Referenzen für anti-TJP1 Antikörper (ABIN675024) Bilder zurück nach oben
Applikationshinweise IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Referenzen für anti-TJP1 Antikörper (ABIN675024) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-TJP1 Antikörper (ABIN675024)

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, 2014

Ruan, Liu, Zhou, Mi, Liu, Wu, Yao, Assaad, Deng, Hou, Wu, Yin: "Chlorogenic acid decreases intestinal permeability and increases expression of intestinal tight junction proteins in weaned rats challenged with LPS." in: PLoS ONE, Vol. 9, Issue 6, pp. e97815, 2014

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, 2012

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, 2011

Bilder

Produktdetails anti-TJP1 Antikörper Target Details TJP1 Anwendungsinformationen Handhabung Referenzen für anti-TJP1 Antikörper (ABIN675024) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Ant...
Immunohistochemistry (IHC) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 ce...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Image was kindly submitted by Sriram Srivatsa from Medical University of Vienna.Paraf...
Western Blotting (WB) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 ce...
'Independent Validation' Siegel
Chargennummer 130913
Validierte Anwendung Immunohistochemistry
Positivkontrolle Testis
Negativkontrolle See Controls section for negative controls
Bewertung Signal was detected in positive control tissue, and not detected in a tissue expected to express very low levels of the target antigen.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Tissues stained came from a human formalin-fixed paraffin embedded (FFPE) tissue microarray (12-003d):
  • Positive control (specimen known to contain the target protein): human testis, which is expected to express high levels of the antigen.
  • Negative Control (specimen known to not contain the target protein): Indeterminate; protein located on cytoplasmic membrane surface of intercellular tight junctions. The protein may be involved in signal transduction at cell-cell junctions. Expression is expected to be widespread. Thymus, expected to express much lower levels of the antigen as compared to testis, is shown to demonstrate a tissue with no detectable expression.
  • Primary antibody isotype control: Testis (specimen known to contain the target protein) treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Testis (specimen known to contain the target protein) treated with secondary antibody only (no primary antibody).
Protokoll
  • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 minutes
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of 95% Ethanol, 3 minutes each - Rinsed in distilled water, 3 changes
  • 1. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 minutes. - Slides were allowed to cool (in citrate) for 30 minutes. - Slides were washed x 3 in Distilled water
  • 1. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 1. Slides rinsed with *reaction buffer* x 3
  • 1. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 minutes
  • 1. Slides rinsed with *reaction buffer*
  • 1. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliters applied/slide) - Ventana Isotype control applied neat - Slides incubated overnight at room temperature (~12 hours ~25°C)
  • 1. Slides rinsed with *reaction buffer* x3
  • 1. *iVIEW Biotinylated IgG* applied and incubated for 8 minutes
  • 1. Slides rinsed with *reaction buffer*
  • 1. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 minutes
  • 1. Slides rinsed with *reaction buffer*
  • 1. *iVIEW DAB/H2O2* applied and incubated for 8 minutes
  • 1. Slides rinsed with *reaction buffer*
  • 1. *iVIEW Copper* applied and incubated for 4 minutes
  • 1. Slides rinsed with *reaction buffer*
  • 1. Slides washed in Dawn Detergent/tap water
  • 1. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 minute - Decolorized (10% Acetic Acid in 70% ethanol), 1 minute - Slides washed in tap water, 1 minute - Bluing (Austin Clear Ammonia), 1 minute - Slides washed in tap water, 1 minute
  • 1. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of Xylene, 5 minutes each - Mounted with Permount
  • 1. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • Tissue Interpretation (limited): - TJP1: Under the staining parameters described above, testis stained weakly (ducts) positive (Figure 1). Substantial signal detected in limited number of other tissues, including: breast, normal (NOS); pancreatic cancer (NOS), and stomach, normal (NOS). Most tissues showed low level of specific signal. Thymus did not have any detectable signal (Figure 4).
  • I-NC (Isotype negative control): No signal detected
  • B-NC (Blank negative control): No signal detected
  • Signal Localization: - Signal to noise was adequate with cytoplasmic, nuclear subcellular localization observed. Rare inner-membrane and no distinct membrane signal observed.
Validierungsbilder
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 1: TJP1 immunostaining of human testis (brown). Counterstain in blue.
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 2: Isotype control immunostaining of human testis (brown). Counterstain in blue.
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 3: Secondary only control immunostaining of human testis (brown). Counterstain...
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 4: TJP1 immunostaining of human thymus (brown). Counterstain in blue.
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 4: Isotype control immunostaining of human thymus (brown). Counterstain in blue.
Immunohistochemistry validation image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1670-1720) antibody (ABIN675024) Figure 6: Secondary only immunostaining of human thymus (brown). Counterstain in blue.