E-Lysis-Solution (RedBloodCell-Lysis for FACS)

Details zu Produkt Nr. ABIN5662621, Anbieter: Anmelden zum Anzeigen
Applikation
Sample Preparation (Prep)
Optionen
Hersteller
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Verwendungszweck Soft and efficiant RED BLOOD CELL Lysis in a 1-step-method for following flow cytometric analysis of whole blood and other samples
Spezifität Erythrocytes lysis, RBC Lysis, Lysing solution, Lysing buffer
Produktmerkmale E-Lysis-Solution is designed for lysing red blood cells (RBC-Lysis) after or before immuno- staining of peripheral blood cells with following flow cytometric analysis and contains appropriate 10x concentrated solution for 1000 tests of 1 mL (1x) each. Red blood cell lysis will be lysed under hypotonic conditions including preservation of leukocytes by mild fixation.
Bestandteile 100 ml E-Lysis-Solution (10x)
Applikationshinweise 100 mL = 1000 tests
Kommentare

- Fast and efficient - RBC-Lysis procedure can be performed in 20 minutes
- Enables immunostaining prior or after RBC-Lysis
- Softly fixed cells with conserved flow cytometric scatter properties
- Specific formulation for reducing background signals
- Quality control assures minimization of lot-to-lot variations

Probenmenge 1 mL
Aufbereitung der Reagenzien

Dilute the 10x stock solution to 1x working solution 1:10 with distilled water (1 part stock solution + 9 parts H2O)

Testdurchführung
  • Add 100 μL of the sample (blood, bone marrow sample or other cell suspension) into your test tube (≥ 5ml). The sample must be completely on the bottom of the tube.
    - For immuno-staining add a sufficient volume (usually 1-20 μL depending on the manufacterer) of antibody solution into the sample, vortex and incubate.
    - Incubation 15 min / RT
    - Add 1 mL of the 1x E-Lysis-Solution to the test tube, vortex and incubate. Repeated vortex rounds within the incubation can optimize the RBC-Lysis
    - Incubation 8-10 min / RT
    - Centrifuge for 5 min at 350g and remove completely the supernatant by aspiration
    - Add 1 mL PBS, vortex and centrifuge for 5 min at 350g and remove completely the supernatant by aspiration
    - Resuspend the pellet in approx. 0.5 mL PBS for direct analysis or in 0.5 mL 0.1 % formaldehyd in PBS for analysis within 24h
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer E-Lysis-Solution contains fomaldehyde, sodium azide and methanol
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung RT
Informationen zur Lagerung Store at 10-25°C
Closed vials: 3 years after delivery
Opened vials: 6 months
Bilder des Herstellers
Flow Cytometry (FACS) image for E-Lysis-Solution (RedBloodCell-Lysis for FACS) (ABIN5662621) Example: Detection of CD4+ and CD8+ lymphocytes in whole blood.