Fluoromount-G™

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Applikation
Fluorescence Microscopy (FM)
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Produktmerkmale Fluoromount-G™ is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. This water-soluble Fluoromount-G™ is used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C. Fluoromount-G™ does not fluoresce and may reduce the amount of fluorochrome quenching during fluorescence microscopy. This medium is supplied as a 1X stock solution containing sodium azide.
Applikationshinweise Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Kommentare

Slide Mounting

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Product is supplied as a 25 mL solution containing 0.1 % NaN 3 as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung RT
Produkt verwendet in: Wang, Wang, Zhang, Shao, Xu, Shi, Shi, Li, Fu, Xue, Lei, Gao, Wang, Gao, Li, Yuan, Zhang: "Genistein sensitizes bladder cancer cells to HCPT treatment in vitro and in vivo via ATM/NF-κB/IKK pathway-induced apoptosis." in: PLoS ONE, Vol. 8, Issue 1, pp. e50175, 2013 (PubMed).

Barbierato, Argentini, Skaper: "Indirect immunofluorescence staining of cultured neural cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 846, pp. 235-46, 2012 (PubMed).

Zang, Christoffersson, Tian, Rashid, Vågesjö, Phillipson, Barg, Tengholm, Welsh: "Aberrant association between vascular endothelial growth factor receptor-2 and VE-cadherin in response to vascular endothelial growth factor-a in Shb-deficient lung endothelial cells." in: Cellular signalling, 2012 (PubMed).

Holmstrand, Sesack: "Projections from the rat pedunculopontine and laterodorsal tegmental nuclei to the anterior thalamus and ventral tegmental area arise from largely separate populations of neurons." in: Brain structure & function, Vol. 216, Issue 4, pp. 331-45, 2011 (PubMed).